RP-HPLC同时测定冷水花属3种植物中2种黄酮苷的含量  

HPLC simultaneous determination of two flavonoids in three kinds of Pilea

作　　者：甘秀海[1] 梁志远[1] 赵超[2] 周欣[2] 

机构地区：[1]贵州师范学院化学与生命科学学院,贵州贵阳550018 [2]贵州师范大学天然药物质量控制研究中心,贵州贵阳550001

出　　处：《云南大学学报（自然科学版）》2015年第1期129-133,共5页Journal of Yunnan University(Natural Sciences Edition)

基　　金：贵州省科学技术基金(黔科合J字[2010]2213号);贵州省科技创新人才团队建设项目(黔科合人才团队(2011)4008);贵州省应用化学特色重点学科(黔教科研发[2012]442号)

摘　　要：建立反相高效液相色谱法同时测定冷水花属湿生冷水花、冷水花、粗齿冷水花植物中木犀草苷和大波斯菊苷2种黄酮类化合物含量的方法.采用Agilent ZORBAX SB-C18（250 mm×Ф4.6 mm,5μm）色谱柱,以乙腈-0.2%乙酸水溶液为流动相进行洗脱,检测波长350 nm,流速0.8 m L·min^-1,柱温25℃.结果木犀草苷和大波斯菊苷分别在2.48～79.36μg·m L^-1、1.68～53.76μg·m L^-1范围内线性关系良好,木犀草苷平均回收率为98.06%,RSD为1.11%;大波斯菊苷平均回收率为99.18%,RSD为0.98%.所建立的冷水花HPLC含量测定方法结果准确可靠,可用于不同品种冷水花植物中木犀草苷和大波斯菊苷的含量测定.To establish an RP-HPLC method for simultaneous determination of luteoloside and cosmosiin in three kinds of Pilea, including Pilea aquarum Dunn, Pilea notate C.H.Wright and Pilea sinofasciata C.J.Chen.Ag- ilent ZORBAX SB-C18（250 mm×Ф4.6 mm,5 μm） column were used to analyze with acetonitrile-0.2% acetic acid aqueous solution as mobile phases.The detective wavelength was at 350 nm.Flow rate was 0.8 mL/min.The column temperature was 25 ℃ .The standard curve of luteoloside and cosmosiin was linear in the range of 2.48—79.36 μg·m L^-1 and 1.68—53.76μg·m L^-1 respectively.The average recovery of luteoloside was 98.06%, RSD was 1.11% ,the average recovery of cosmosiin was 99.18% ,RSD was 0.98%.The method is precise,reliable and suitable for simultaneous determination of luteoloside and cosmosiin in three kinds of Pilea.

关 键 词：冷水花 反相高效液相色谱 木犀草苷 大波斯菊苷 含量测定 

分 类 号：R917[医药卫生—药物分析学]