无机离子对嗜盐放线菌株YIM90615代谢产物多样性的影响  

The influences of inorganic ions on the diversities of secondary metabolites produced by Actinopolyspora YIM 90615

作　　者：张正芸 陆晓菊 官会林[1] 唐蜀昆[2] 丁章贵[2] 

机构地区：[1]云南师范大学能源与环境科学学院,云南昆明650092 [2]云南大学微生物研究所,云南昆明650091

出　　处：《云南大学学报（自然科学版）》2015年第1期147-154,共8页Journal of Yunnan University(Natural Sciences Edition)

基　　金：国家自然科学基金(41361075);云南省应用基础研究重点项目(2013FA015);云南省教育厅科学研究重大专项(ZD2013008)

摘　　要：为了研究Na Cl浓度、金属离子(Cr7+、Mg2+及K+)、氯化钪及卤素(I-、Br-及F-)对嗜盐放线菌株YIM90615液体发酵过程中次级代谢产物多样性的影响,作者采用HPTLC(high performance thin layer chromatography)技术,结合紫外(254 nm)检测、碘及硫酸-茴香醛显色方法,对该菌代谢产物的HPTLC图谱进行了定性分析.结果显示,Na Cl添加质量浓度为100 g/L时该菌株发酵效果最佳,有利于其代谢产物的多样性及量的积累;Cr7+和K+有利于其次生代谢产物的产生;1 g/L的氯化钪能增加其次生代谢产物的丰富性;F-对其次生代谢产物多样性有较好的促进作用.试验结果表明外源添加一定浓度和种类的盐离子有助于提升YIM 90615液体发酵过程中次生代谢产物多样性,为新型抗生素及其它有用化合物的产生创造合适条件,同时有助于嗜盐放线菌发酵培养基的筛选工作.To understand the effect of salt irons, such as NaCl, metal irons （ Cr^7＋, Mg^2＋, and K^＋ ）, SeCl3, and halogen （I^-, Br^-, and F^-） ,on the diversity of secondary metabolites produced by Actinopolyspora YIM 90615, HPTLC chromatographed by UV254, iodine and anisaldehyde-sulfuric acid were performed.The results showed that application of NaC1 at the final concentration of 100 g/L showed the best promotion to the growth of the strain and the diversity of secondary metabolites production, and Cr^7＋ , K^＋ also help to produce more secondary metabo- lites, additional 1 g/L ScCl3 and F- could promote the diversity of secondary metabolites too.These results sugges- ted that exogenous application of salt irons could obviously stimulate the growth of strain YIM 90615 and the pro- ductivity and diversity of second metabolisms, which could provide the theoretical basis for the practical applica- tion of Actinopolyspora in future and create suitable conditions for producing new antibiotics and other useful com- pounds.It also provides theoretical basis for the selection of the fermentation medium for other Actinomycetes.

关 键 词：嗜盐放线菌 液体发酵 次级代谢产物 高效薄层色谱(HPTLC) 

分 类 号：Q93-335[生物学—微生物学] Q935