二倍体马铃薯原生质体培养与植株再生  

Culture and Plantlets Regeneration from Protoplast of Diploid Potato

作　　者：刘文萍[1] 刘建新[1] 南相日[1] 张丽娟[2] 盛万民[2] 张举梅[3] 

机构地区：[1]黑龙江省农业科学院生物技术研究所,黑龙江哈尔滨150086 [2]黑龙江省农业科学院作物育种研究所,黑龙江哈尔滨150086 [3]黑龙江省农业科学院对俄农业技术合作中心,黑龙江哈尔滨150086

出　　处：《黑龙江农业科学》2015年第1期6-9,共4页Heilongjiang Agricultural Sciences

基　　金：科技部国家国际科技合作资助项目(2011DFR30840)

摘　　要：原生质体培养是体细胞杂交的基础,同时也是获得变异材料的重要手段。以引进的4个二倍体马铃薯为材料,研究了渗透浓度、酶液组成、酶解时间及材料预处理对原生质体产量和活力的影响。结果表明：预处理是原生质体成活、分裂及后期发育的重要条件,处理D预处理效果最好,原生质产量和活力最高,且有较多细胞分裂;马铃薯原生质体适宜的渗透浓度为0.35-0.40mol·L^-1;RH2和RH3酶解时间以4h为宜,RH1和RH4酶解时间以4.5h为宜;酶解液为2.0%纤维素酶和0.2%果胶酶的混合液。基因型对原生质体培养影响较大。经过培养,有2个二倍体材料获得了再生植株。Protoplast culture is the foundation of somatic hybridization and an important method to acquire mu‐tant material .Taking four introduced diploid potato as materials ,the effect of osmotic concentration ,enzymoly‐sis conduction ,enzymolysis time and pretreatment on yield and vitality of protoplast were studied .The results indicated that pretreatment was a key factor for surviving ,division and development of protoplast .The proto‐plast yield and vitality of treatment D were the best .The osmotic concentration suitable for protoplast was 0 .35-0 .40 m·L^‐1 .The suitable enzymolysis time for RH2 and RH3 was 4 h ,RH1 and RH4 was 4 .5 h .Enzy‐matic hydrolysate was 2 .0% Cellulase RS Onozuka and 0 .2% Pectolyase Y‐23 .The protoplast culture was greatly influenced by genotypes .Regeneration plantlets of two diploid potato materials were successfully gained .

关 键 词：二倍体马铃薯 原生质体 再生植株 

分 类 号：S532[农业科学—作物学]