密楝果实中绿原酸含量的测定  

Determination of Chlorogenic Acid in Evodia lenticellata Huang

作　　者：王星[1] 张娜[1] 赵桦[1] 

机构地区：[1]陕西理工学院生物科学与工程学院,陕西汉中723000

出　　处：《黑龙江农业科学》2015年第1期112-115,共4页Heilongjiang Agricultural Sciences

基　　金：陕西省教育厅专项科研计划资助项目(12JK0842);陕西理工学院研究生创新基金资助项目(SLGYCX1312)

摘　　要：为了进一步开发利用密楝资源,以50%甲醇溶液为溶剂,超声提取法从密楝果实中提取绿原酸,高效液相色谱法检测提取液中绿原酸含量。提取条件为：超声功率240 W,超声频率40kHz,提取温度60℃,超声提取30min。色谱条件为：色谱柱为Inertsil ODS-3C18（4.6×150mm,5μm）柱,以乙腈-0.4%磷酸水溶液（8：92）为流动相,流速1.0mL·min^-1,波长327nm,柱温30℃。绿原酸在20.8-104μg·mL^-1范围内与峰面积呈现良好的线性关系,Y=32 304X-44 968（R^2=0.999 9）,平均加样回收率99.59%,RSD为1.69%（n=6）。密楝果实中含有丰富的绿原酸成分,其含量随产地的变化有一定的差异,变化范围在15.34-11.91mg·g^-1。建立的密楝果实中绿原酸含量检测方法简便、准确、重复性好,可用于密楝药材的质量控制。In order to further develop and utilize Evodia lenticellata Huang resources ,the chlorogenic acid were extracted from E .lenticellata Huang using 50% methanol ,and the conditions of the ultrasonic wave as follows：extraction ultrasonic power of 240 W ,ultrasonic frequency of 40 kHz ,temperature of 60℃ and ultrasonic treat‐ment time of 30 min .Samples were analyzed on an Inertsil ODS‐3C18 column（4 .6 × 150 mm ,5μm） using aceto‐nitrile‐0 .4% phosphoric acid（8∶92）as mobile phase ,speed of flow was 1 .0 mL·min^‐1 ,the examination wave length was 327 nm and the column temperature was 30℃ .The chlorogenic acid showed a good linearity in the range of 20 .8-104 μg·mL^‐1 ,egression equation was Y=32 304X -44 968（R^2 =0 .999 9）and the average re‐covery was 99 .59% （RSD=1 .69% ,n=6） .The chlorogenic acid is richer in E .lenticellata Huang and there are some different in the content of chlorogenic acid in E .lenticellata Huang among different origins .The estab‐lished method is simple ,credible ,accurate ,repeatable and culd be used for the quality control of E .lenticellata H uang .

关 键 词：密楝果实 绿原酸 含量测定 

分 类 号：S567.19[农业科学—中草药栽培]