巨魾野生群体遗传多样性的RAPD分析  

RAPD analysis of genetic diversity in the wild populations of Bagarius yarrelli

作　　者：杜民[1] 牛宝珍[1] 罗彩艳[1] 刘艳红[1] 

机构地区：[1]红河学院生命科学与技术学院,云南省高校农作物优质高效栽培与安全控制重点实验室,云南蒙自661199

出　　处：《淡水渔业》2015年第1期15-19,24,共6页Freshwater Fisheries

基　　金：国家自然基金(31360638);云南省教育厅科学研究基金重大专项项目(ZD2013009);红河学院中青年学术带头人后备人才项目(2014HB0203);红河学院博士专项(14bs11)

摘　　要：巨魾(Bagarius yarrelli)为云南特有的经济鱼类,利用随机扩增多态性DNA(RAPD)技术对元江下游的河口巨野生群体进行了遗传多样性分析,在50条随机引物中筛选出19条多态性较好的引物,通过PCR扩增,19条引物在巨群体中共检测到67个位点,其中多态性位点66个,平均多态性位点比率为98.51%,个体间平均遗传相似系数为0.890 9,个体间平均遗传距离为0.117 6;群体Nei's基因多样性指数为0.291 6,Shannon信息指数为0.426 9,结果表明巨野生群体具有较高的遗传多样性。Bagarius yarrelli is an ecomomic endemic fish in the Red River , in Yunnan Province, China.The genetic di-versity of B.yarrelli populations from Hekou was studied by random amplified polymorphic DNA （RAPD） technology.19 polymorphic primers were screened in 50 random primers by PCR（Polymerase Chain Reaction）, 67 loci were detected in B.yarrelli fish groups, of which 66 loci were polymorphic loci, the average ratio was 98.51%, The average genetic simi-larity index and average genetic distance were 0.890 9 and 0.117 6, respectively; The Nei′s gene diversity index was 0.291 6 and shannon′s information index was 0.426 9.The results showed that there was a high genetic diversity of the Hekou B.yarrelli.The wild B.yarrelli population genetic diversity through RAPD analysis can provide reference for the ra -tional development and protection of B.yarrelli fish germplasm resources in the future.

关 键 词：巨魾(Bagarius yarrelli) RAPD 引物筛选 遗传多样性 

分 类 号：S917.4[农业科学—水产科学]