氯霉素抗原的制备及其在免疫胶体金快速检测中的应用  

Preparation of Chloramphenicol Antigen and its Application of Rapid Detection of Chloramphenicol Residue by Colloidal Goad-based Immunochromatography

作　　者：潘嘉慧[1] 田峻[1] 赵亮亮[1] 沈国权[1] 朱海云[1] 

机构地区：[1]佛山市质量计量监督检测中心,广东佛山528225

出　　处：《广东化工》2015年第1期39-40,共2页Guangdong Chemical Industry

基　　金：佛山市禅城区科技开发专项资金[2012A1049]

摘　　要：本研究从优化氯霉素免疫抗原、包被抗原的结构及优化偶联技术角度出发,提高氯霉素抗体的特异性、亲和力,并通过优化样品前处理步骤,最终得到氯霉素胶体金免疫层析试纸条的检测低限为0.1μg/L,特异性好,稳定性高。The study optimized the structure of chloramphenicol immune antigen, envelope antigen and the coupling technology, improving the specificity of antibody of chloramphenicol, affinity, and simplifying the sample preparation procedures. The results showed that the rapid detection limit of chloramphcnicol residue by colloidal goad-based immunochromatography can reach 0.1 μg/L, showing good specificity and high stability.

关 键 词：氯霉素抗原 免疫胶体金 快速检测 

分 类 号：TQ465.3[化学工程—制药化工]