高效脱硫菌At.f菌的化学诱变育种研究  

Research on Chemical Mutagenesis Bbreeding of the High Desulfurization Bacterium At.f

作　　者：方力[1] 李茹[1] 唐家彬[1] 王欢[1] 

机构地区：[1]西安工程大学环境工程,陕西西安710048

出　　处：《广东化工》2015年第2期12-13,19,共3页Guangdong Chemical Industry

摘　　要：本实验通过使用1-甲基-3-硝基-1-亚硝基胍(NTG)诱变处理对数期嗜酸氧化亚铁硫杆菌(简称At.f菌),研究了同一时间段内与不同时间段内的不同浓度的诱变剂和不同诱变处理时间对诱变效应的影响,并在此基础上对诱变后菌株的多聚糖(简称EPS)变化率进行了探究。实验结果显示诱变后菌株的氧化活性在原始菌株的基础上提高约为5倍,诱变后的At.f菌的EPS变化率增加,反应了其氧化活性的增加。此外,通过诱变使得菌种的培养周期大大缩短。In the experiment, 1-methyl-3-nitro-1-nitrosoguanidine （NTG） was used for the mutagenic treatment of Acidthiobacillus ferrooxidans （At.f） at its logarithmic phase, and the influence of different concentrations of mutagen on the same time and different time intervals and different treatment time on the mutagenic effect were studied. In addition, tile rate of changes of the extracellular polysaccharides （EPS） on the modified strain was studied on the basis of the above. The experimental results show that the oxidation activity of the modified strains based on the original strain is increased about 5 times, and the rate of changes of EPS on the modified At.f is increased also indicated the increase of the oxidation activity of At.f. In addition, the training cycle of the strain is greatly shortened by mutation.

关 键 词：诱变处理 EPS变化率 氧化亚铁硫杆菌 

分 类 号：Q93-3[生物学—微生物学]