建立小鼠异位心脏移植模型的心得  

Establishment of heterotopic cardiac transplantation model in mice

作　　者：雷彪[1] 何松青[1] 

机构地区：[1]桂林医学院附属医院肝胆胰外科,桂林541001

出　　处：《国际外科学杂志》2014年第12期834-836,共3页International Journal of Surgery

摘　　要：目的 建立小鼠异位心脏移植模型,为进行移植免疫研究提供动物模型.方法 昆明（KM）小鼠50只,随机分供、受体两组,借鉴大鼠异位心脏移植Ono式模型经验,并加以改良,将供体升主动脉、肺动脉分别与受体的腹主动脉和下腔静脉行端侧连续吻合.结果 成功建立了小鼠腹腔异位心脏移植模型,实验成功率为84％ （21/25）.供、受体的手术时间分别为（11.0±l.0）min和（60.0 ±2.0）min.结论 熟练掌握显微外科技术后,可以成功建立小鼠异位心脏移植模型.Objective To establish a mouse model of heterotopic heart transplantation and construct an animal model for the study of transplantation immunity.Methods According to the surgical procedures of Ono＆#39;s pattern in mice cardiac heterotopic transplantation model,50 Kunming mice were divided into donors and recipients randomly.The donor heart aorta and the recipient ventral aorta,the donor pulmonary artery and the recipient inferior caval vein,were anastomosed by using the end-to-side suture technique respectively.Results The mouse model of heterotopic heart transplantation was established successfully with a success rate of 84% （21/25）.The average time of donor operation and recipient operation was （11.0 ± 1.0） min and（60.0 ± 2.0） min respectively.Conclusions In the base of mastering the technique of operation facility,the model of mice heart heterotopic transplantation would be established successfully.

关 键 词：小鼠 腹部 心脏移植 模型 

分 类 号：R654.2[医药卫生—外科学]