促红细胞生成素对新生大鼠缺氧缺血性脑损伤后VEGF及NF-κB表达的影响  

Effect of erythropoietin on vascular endothelial growth factor and nuclear factor kappa B expression in neonatal rats after hypoxic-ischemic brain damage

作　　者：朱迪卿[1,2] 庞高峰[1,2] 

机构地区：[1]常州市第一人民医院 [2]苏州大学附属第三医院儿科,江苏常州213003

出　　处：《中国儿童保健杂志》2015年第1期42-45,共4页Chinese Journal of Child Health Care

摘　　要：目的对7日龄缺氧缺血性脑损伤(hypoxic ischemic brain damage,HIBD)大鼠进行促红细胞生成素干预,并对血管内皮生长因子(vascular endothelial growth factor,VEGF)及核因子-κB(nuclear factor kappa B,NF-κB)表达的变化情况进行检测分析。方法将75只SD新生大鼠按照随机数字表分为三组:1)假手术组取颈部正中切口,接着游离左侧颈总动脉,并进行切口缝合;2)HIBD组分离左侧颈总动脉,接着游离血管并行结扎,缝合后放入含氧量为8%的环境中2h;3)EPO处理组游离并结扎左侧颈总动脉,随后放于含氧量为8%的环境中2h,并腹腔内注射rhEPO 1次(剂量:5 000U/kg)。于6、12、24、72h后分别留取脑标本,并对脑组织中VEGF及NF-κB的表达情况进行检测。结果 1)与假手术组对比,HIBD组和EPO处理组中VEGF的表达有明显增高(P<0.05);2)HIBD组NF-κB表达高于假手术组(P<0.05),HIBD组高于EPO处理组(P<0.05)。结论促红细胞生成素有增高VEGF蛋白表达、降低NF-κB蛋白表达作用,这一机制可能对新生大鼠缺氧缺血性脑损伤起保护作用的机制之一。Objective To investigate the effects of erythropoietin on vascular endothelial growth factor（VEGF）and nuclear factor kappa B（NF-κB）expression in neonatal rats with hypoxic-ischemic brain damage. Methods The 7days old rats were randomly divided into three groups（n=24in each group）：sham-operated group,HIBD group and EPO-treated group.In sham-operated group,the median incision of neck was performed,and left common carotid artery was isolated,but not hypoxia-ischemia.In HIBD group,left common carotid artery was isolated and ligated,then the models were prepared under hypoxia for 2h.In EPO-treated group,the model rats were instantly given single intraperitoneal injection of rhEPO（5 000U/kg）.The expression of VEGF and NF-κB were detected by immunohistochemistry at 6,12,24,72 hours after the operation. Results 1）Expression of VEGF was higher in HIBD group than in sham-operated group.Expression of VEGF was significantly higher in EPO-treated group than in sham-operated group and HIBD group at the same time（P〈0.01）.2）Expression of NF-κB was higher in HIBD group than in sham-operated group.Expression of NF-κB was higher in HIBD group than in EPO-treated group at the same time（P〈0.01）. Conclusion Erythropoietin can up-regulate expression of VEGF and reduce expression of NF-κB in neonatal rats after hypoxic-ischemic brain damage,which may be one of the mechanisms for protecting HIBD.

关 键 词：促红细胞生成素 血管内皮生长因子 核因子-ΚB 缺氧缺血 新生大鼠 

分 类 号：Q954.5[生物学—动物学]