兔肝脏和肠道中戊型肝炎病毒抗原的检测及组织病理学观察  

Detection of the Hepatitis E virus(HEV) and Its Histopathologic Observation in Liver and Intestinal tract of Rabbit

作　　者：郭兆杰[1] 佘锐萍[1] 毛晶晶[1] 王铜铜 赵月[1] 肖鹏[1] 陈建[1] 马龙欢 

机构地区：[1]中国农业大学动物医学院,北京海淀100193

出　　处：《中国兽医杂志》2014年第11期19-20,23,I0003,共4页Chinese Journal of Veterinary Medicine

基　　金：国家自然科学基金项目(31272515;31072110)

摘　　要：为探讨兔体内戊型肝炎病毒(HEV)的感染情况,本试验从河北某屠宰场采集了136例兔肝脏样品和101例肠道样品。采用免疫组织化学染色方法对上述样品中的HEV抗原进行了检测,同时应用H.E.染色和Mallory三色染色法对其病理形态学变化进行了观察。研究结果显示,136例肝脏中HEV抗原免疫组织化学阳性样品共有96例,阳性率为70.59%,101例肠道中HEV抗原免疫组织化学阳性样品共有79例,阳性率为78.22%。组织病理学观察可见肝脏和肠道均有明显的炎症变化。本研究结果表明,兔群中存在较高的HEV感染率。The objective of this study was to investigate the prevalence of Hepatitis E Virus（HEV）infection in rabbits,136 liver samples and 101 intestinal tract samples were collected from slaughter-houses in Hebei. Immunohistochemical staining was carried out to detect HEV Ag in these samples,and the pathological morphological changes were observed by using H.E. staining and Mallory trichromatic staining. Results showed that 96 out of 136 liver samples were immunohistochemical positive for HEV Ag,the positive rate was 70.59%,and 79 out of 101 intestinal tract samples were immunohistochemical positive for HEV Ag,the positive rate was78.22%.Obvious changes of inflammation could be seen both in liver and intestinal tract through histopathological observation. The results indicate that there was a high infection rate of HEV in rabbits.

关 键 词：兔 戊型肝炎 免疫组织化学 组织病理学 

分 类 号：S852.166[农业科学—基础兽医学]