HPLC法测定银杏叶缓释微丸胶囊中山奈素、异鼠李素、槲皮素的含量  

Determination of the Content of Haempferol,Isorhamnetin and Quercetin in Folium Ginkgo Pellets Capsules by HPLC

作　　者：吴杏梅[1] 谢燕萍[1] 陈楚裕[1] 刘金瑶[1] 

机构地区：[1]广东省佛山市第一人民医院,佛山528000

出　　处：《北方药学》2015年第1期17-18,共2页Journal of North Pharmacy

摘　　要：目的：建立以高效液相色谱法测定银杏叶缓释微丸胶囊中山奈素、异鼠李素、槲皮素含量的方法。方法：以C18为色谱柱,流动相为甲醇∶0.4%磷酸溶液（45∶55）,检测波长为360nm,流速为1ml/min,柱温为30℃,进样量是10μl。结果：槲皮素在0.1505~0.9030μg（r=0.9999）浓度范围内有良好的线性关系,平均回收率为99.86%,RSD=0.4%;山奈素在0.1450~0.8940μg（r=0.9998）浓度范围内有良好的线性关系,平均回收率为99.79%,RSD=0.5%;异鼠李素在0.0995~0.5970μg（r=0.9998）浓度范围内有良好的线性关系,平均回收率为99.80%,RSD=0.3%。结论：本方法简便、准确、专属性强,可用于该制剂的质量控制。Objective： To establish a method for the determination of haempferol, isorhamnetin and quercetin in folium ginkgo pellets capsules Methods： HPLC was performed on a C18 column using mathanol∶0.4%H3PO4（45∶55）as the mobile phase at a flow rate of 1ml/min. The detection wavelength was at 360 nm. The column temperature was 30℃ and the sample size was 10μl. Results： The linear range of quercetin was 0.1505 ~0.9030μg（r =0.9999）,the average recovery was 99.86%（RSD =0.4%）;The linear range of haempferol was0.1450~0.8940μg（r=0.9998）,the average recovery was 99.79%（RSD=0.5%）;The linear range of isorhamnetin was 0.0995~0.5970μg（r=0.9998）,the average recovery was 99.80%（RSD=0.3%）;Conclusions： The method is simple,accurate,special and can be used for the quality control of folium ginkgo pellets capsules.

关 键 词：银杏叶缓释微丸胶囊 山奈素 异鼠李素 槲皮素 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]