新生大鼠重复吸入亚麻醉浓度七氟醚对成年期学习记忆能力的影响  

Effects of repeated inhalation of sevoflurane under sub-anesthetic concentration in neonatal period of rats on capability of learning and memory in adult period

作　　者：郑淑文[1,2] 朱昭琼[1] 张超[1] 朱宇航[1] 

机构地区：[1]遵义医学院附属医院麻醉科,遵义563003 [2]商丘市第一人民医院麻醉科,商丘476100

出　　处：《郑州大学学报（医学版）》2014年第5期651-653,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家自然科学基金项目81360180

摘　　要：目的：探讨新生期重复吸入亚麻醉浓度七氟醚对大鼠成年期学习记忆能力的影响。方法：24只7日龄SD大鼠随机分为2组，每组12只。实验组分别于出生7、14、21 d吸入体积分数1．5％七氟醚1 h，对照组吸入运载气体，于第91～95天行定位航行和空间探索实验，记录逃避潜伏期、原平台象限滞留时间百分比、原平台象限运动距离百分比及原平台象限进入次数百分比；于第96～104天行不固定平台实验，记录成功到达平台的次数和达到标准所用总时间。结果：与对照组相比，实验组逃避潜伏期延长，原平台象限滞留时间百分比及运动距离百分比减小（F组间＝41．297，t＝3．421和3．122，P均＜0．05）；2组大鼠成功到达平台的次数及达到标准所用总时间比较，差异无统计学意义（F组间＝2．184和1．143，P均＞0．05）。结论：新生期重复吸入亚麻醉浓度七氟醚会损害大鼠成年期学习记忆能力，而对工作记忆能力无影响。Aim：To investigate the effects of repeated inhalation of sevoflurane under sub -anesthetic concentration in the neonatal period of rats on capability of learning and memory in the adult period .Methods：Seven-day old SD rats were randomly allocated into 2 groups,experimental group（n=12,group S） and control group（n=12,group C）.The rats in group S were anesthetized with 1.5%（ V/V） sevoflurane for 1 h on the 7th,14th and 21st d after birth respectively ,while group C inhaled carrier gas instead .Place trial and probe trial were carried out on the 91st-95th d after birth,and the es-cape latency ,the residence time ,movement distance and the number of entering into the platform area were recorded .Learn-ing to reach criterion test was carried out on the 96th-104th d after birth,and the number of entering into the platform and the time used to reach the criteria were recorded .Results：Compared with group C ,the escape latency of group S was lon-ger,the residence time and movement distance were shorter （Fgroup =41.297,t=3.421 and 3.122,P〈0.05）.The number of entering into the platform and the time used to reach the criteria had no significant differences between the 2 groups （Fgroup =2.184 and 1.143,P〉0.05）.Conclusion：Repeated inhalation of sevoflurane under sub-anesthetic concentration during the neonatal period may injure the long-term learning and memory ability of rats ,while have no effects on working memory capability .

关 键 词：七氟醚 学习记忆 新生大鼠 成年期 

分 类 号：R726[医药卫生—儿科]