雌、孕激素下降对大鼠肾脏组织中水通道蛋白表达的影响  

Effects of reduced estrogen and progesterone on renal aquaporin expression in rats

作　　者：卫红[1] 王贵宪[2] 徐纪中[2] 袁维堂[2] 卫军[3] 

机构地区：[1]河南司法警官职业学院实验中心,郑州450000 [2]郑州大学第一附属医院肛肠外科,郑州450052 [3]武警河南总队医院感染管理科,郑州450052

出　　处：《郑州大学学报（医学版）》2014年第5期734-737,共4页Journal of Zhengzhou University(Medical Sciences)

摘　　要：目的:探讨卵巢切除后激素水平变化对肾脏组织水通道蛋白(AQP)表达和水代谢的影响。方法:60只大鼠随机分为2组。卵巢切除组大鼠切除卵巢后分别饲养0、2和4周(n=10);对照组大鼠只显露卵巢和输卵管,但不结扎切除,分别饲养0、2和4周(n=10)。分别收集2组尿、血液,取出肾脏,检测尿、血液渗透压,检测血浆雌、孕激素和血管加压素水平,免疫印迹和免疫组化检测肾脏组织AQP2、AQP1和AQP3的表达。结果:大鼠切除卵巢后2周,雌激素及孕激素水平均下降,血管加压素水平升高,体重增加,尿量减少,肾脏组织AQP2、AQP1和AQP3的表达上升(P均<0.05)。手术后4周,上述变化更加明显(P均<0.05)。结论:雌、孕激素水平下降可能通过血管加压素水平升高造成肾脏AQP高表达,进而导致尿量减少和体重增加。Aim：To investigate the effects of decreased estrogen and progesterone on alteration of renal aquaporin （ AQP） and water retention after ovariectomy （ OVX） .Methods：A total of 60 rats were divided into 2 groups randomly .The expressions of AQP1,AQP2 and AQP3 in the kidney were examined at 0,2 and 4 weeks after operation in the two groups . Results：A persistent and significant increase in AQP 1,AQP2 and AQP3 expressions in the kidney at 2 and 4 weeks after OVX was observed （ P〈0.05 ） .Plasma levels of estrogen and progesterone decreased ,whereas plasma AVP level was signif-icantly increased at 2 and 4 weeks after OVX（P〈0.05）.Urine output was significantly reduced while the weight of the rats was elevated at 2 and 4 weeks after OVX（P〈0.05）.Conclusion：Decreased estrogen and progesterone may contribute to the upregulation of renal AQP1,AQP2 and AQP3 through AVP pathway.The upregulation of AQP may play an important role in the decreased urine output and weight-loss after OVX.

关 键 词：水通道蛋白 雌激素 卵巢切除 孕激素 大鼠 

分 类 号：R692[医药卫生—泌尿科学]