家蚕肠道细菌菌落形态与产消化酶相关性研究  

On Intestinal Bacteria Colony Morphology of Silkworm and Enzyme-Producing Correlation

作　　者：李冠楠[1] 夏雪娟[2] 赵欢欢[1] 隆耀航[1] 唐文超[1] 辛佳[1] 李汉歌 朱勇[1] 

机构地区：[1]西南大学生物技术学院,重庆400716 [2]西南大学食品科学学院,重庆400716

出　　处：《西南师范大学学报（自然科学版）》2014年第12期53-57,共5页Journal of Southwest China Normal University(Natural Science Edition)

基　　金：国家农业科技成果转化资金(2012GB2F100376)

摘　　要：【目的】研究肠道细菌的菌落形态与其产酶的相互关系,有助于产酶菌的筛选和家蚕饲用微生态制剂的开发应用.【方法】从家蚕肠道内共分离出细菌28株,挑选菌落形态有明显差异的细菌7株用于产酶菌研究.用筛选培养基鉴别各菌株的产酶情况,并结合16SrDNA序列测定和系统发育分析法鉴定其种属.根据菌落形态和产酶特性,推测细菌的表型基因与产酶基因表达之间存在某种相互联系.【结果】鉴定出产酶的细菌为芽孢杆菌、葡萄球菌、不动杆菌,这些细菌在菌落形态上有一定的相似性,菌落均为乳白色、生长速度快、表面粗糙、边缘不整齐.【结论】细菌菌落形态差异性可作为产酶菌筛选的参考依据,减少实验工作量和节省时间等.为筛选益生菌和饲用微生态制剂的研发提供理论基础.The relationship of intestinal bacteria colony morphology and enzyme production has been stud‐ied to help screen enzyme‐producing and microbial ecological agents in development and application .The 28 strains intestinal bacteria of silkworm have been isolated ,and the colony morphology of 7 stains bacteria have differences which have been used enzyme‐producing research . The enzyme‐producing situation of strains have been identified by screening medium ,and the strains have been identified species by means of phylogenetic analysis according to the analysis of the 18S rDNA sequencing .With the characteristic of col‐ony morphology and enzyme‐producing situation ,suggesting that phenotypic gene of the bacteria and en‐zyme‐producing gene of the bacteria exist certain relation in expression .The enzyme‐producing bacteria are Bacillus ,Staphylococcus ,Acinetobacter ,and these bacteria have a certain similarity in colony morphology which they have white colonies ,fast growth ,rough surface ,and jagged edge .It is concluded that colony mor‐phology differences in enzyme‐producing bacteria can be used as a reference for screening to reduce experimental workload and save time .For screening and probiotics provide a theoretical basis for application and development .

关 键 词：肠道 菌落形态 产酶菌 鉴定 微生态制剂 

分 类 号：S881.2[农业科学—特种经济动物饲养]