化学发光免疫法检测梅毒特异性抗体结果分析  

Detection of Syphilis Specific Antibody by CLIA

作　　者：张迎梅[1] 徐小平[1] 杨玉芹[1] 胡玲[1] 

机构地区：[1]南京医科大学附属淮安第一医院,江苏淮安223000

出　　处：《标记免疫分析与临床》2014年第6期740-742,共3页Labeled Immunoassays and Clinical Medicine

摘　　要：目的探讨化学发光法检测梅毒螺旋体特异性抗体的应用价值。方法应用化学发光免疫测定法检测12503例住院患者梅毒螺旋体特异性抗体,并同ELISA、TPPA、RPR方法进行比较。结果 12503例住院患者化学发光法检出梅毒螺旋体特异性抗体阳性308例,同ELISA方法比较,阳性符合率99.08%,阴性符合率99.98%,总符合率99.93%。二者检测结果高度一致。同TPPA比较,阳性符合率98.7%。结论化学发光免疫法检测梅毒特异性抗体具有特异性好、灵敏度高、结果易判断、便于自动化等优点,适合于梅毒的筛查。Objective To evaluate the application value of CLIA for detection of syphilis specific antibody. Methods 12503 serum samples were detected by CLIA. The detection results of CLIA were compared with detection with ELISA or TPPA and RPR. Results In 12503 serum samples, 308 of them were positive detected with CLIA, while 311 samples were positive detected with ELISA. There was an agreement of 99.93 % between CLIA and ELISA. Compared with TPPA method, the positive coincidence rate was 98.7%. Conclusion CLIA is easy to perform and may be suitable for large-scale screening of syphilis specific antibody.

关 键 词：梅毒螺旋体 抗体 化学发光免疫测定法 

分 类 号：R446.6[医药卫生—诊断学] R759.1[医药卫生—临床医学]