不同实验顺序和不同厂家试剂对测定尿酸的干扰因素分析和解决方案  

The Interference Factors in Determination of Uric Acid with Automatic Biochemistry Analyzer

作　　者：刘鹏年[1] 郑运周 杨硕 

机构地区：[1]河北省涿州市医院检验科,河北涿州072750 [2]石家庄和平医院,河北石家庄050082 [3]保定第二中心医院,河北保定071028

出　　处：《标记免疫分析与临床》2014年第6期746-748,共3页Labeled Immunoassays and Clinical Medicine

摘　　要：目的探讨全自动生化分析仪测定尿酸(UA)时产生交叉污染的来源及避免对策。方法筛查出对UA测定存在污染的试剂;确定干扰试剂的种类后进行试剂污染试验,检测交叉污染的情况。结果在试剂干扰检测中发现,高密度脂蛋白胆固醇(HDL-C)测定对UA的测定存在污染;试剂污染试验发现,HDL-C对UA检测结果有明显的正干扰。结论全自动生化分析仪测定UA会存在项目间的交叉污染,可通过调整项目检测顺序、建立试剂针特殊清洗程序,或者更换具有抗污染成分的试剂等方法避免交叉污染。Objective To investigate the sources of cross-contamination in the determination of uric acid with automatic biochemical analyzer. Methods The contamination reagent in the determination of UA were Screened out. After determining the interference reagent, the cross-contamination tests were performed. Results In combination detection, HDL measurement had contamination in the UA measurement. The reagent contamination trial found a significant positive interference of HDL reagent to UA test results. Conclusion Automatic biochemical analyzer has cross-contamination in the determination of uric acid. The cross-contamination could be reduced and avoided by adjusting the project detection order, establishing special cleaning procedures for reagent needle, or by replacing the reagents with anti-contamination ingredients.

关 键 词：生化试剂 尿酸 交叉污染 

分 类 号：R446.1[医药卫生—诊断学]