基于psbA-trnH条形码的毒品原植物大麻及其混伪品的鉴别  

Identification of cannabis and adulterants using psbA-trnH barcoding

作　　者：宋炳轲 冯涛[2] 杨雪莹[3] 裴黎[3] 张颖[3] 徐小玉[3] 涂政[3] 

机构地区：[1]中国人民公安大学,北京100038 [2]四川省成都市公安局物证鉴定所,610017 [3]公安部物证鉴定中心,北京100038

出　　处：《刑事技术》2014年第6期39-41,共3页Forensic Science and Technology

摘　　要：目的利用叶绿体上的间隔区psbA-trnH条形码序列鉴别毒品原植物大麻及其混伪品,为毒品原植物大麻的鉴定提供新方法。方法采用PCR法扩增psbA-trnH间隔序列,双向测序后运用CodonCode Aligner、MEGA5.1软件进行数据处理,构建系统聚类树(NJ树)。结果 psbA-trnH条形码序列分析表明大麻种内与种间遗传距离具有较大差异,基于psbA-trnH条形码构建的NJ树可鉴别大麻及其混伪品。结论 psbA-trnH序列可以作为鉴定毒品原植物大麻及其混伪品的候选条形码序列,为毒品原植物大麻的快速、准确鉴定提供了新方法;DNA条形码技术在犯罪案件中涉及的植物鉴定中具有极大的应用前景。Objective The psbA-trnH sequence of cpDNA was utilized as a novel technique to discriminate drug original plants of cannabis and adulterants. Methods The psbA-trnH regions were amplified and sequenced bi-directionally. Then the obtained sequences were assembled using the CodonCode Aligner. The psbA-trnH genetic distances of the regions were computed in accordance with the kimura 2-parameter （K2P） model and Neighbor-joining （NJ） phylogenetic trees were constructed using MEGA5.1. Results The psbA-trnH sequences analysis showed that K2P distances of inter-and intra-specific were different significantly. The NJ trees showed that the cannabis could be distinguished easily from adulterants. Conclusions The psbA-trnH fragment could be used as a candidate barcode for identifying cannabis from adulterants and it provide a new method to accurately identify cannabis. DNA barcoding has broad prospects for plant identification in criminal investigation.

关 键 词：法医遗传学 大麻 DNA条形码 毒品 

分 类 号：Q78[生物学—分子生物学]