磁珠法对混合斑中精子DNA批量自动提取的应用研究  

Automatic extraction of sperm DNA from mixed stain samples using DNA IQTMSystem

作　　者：王凤宽[1] 杜舟[1] 王传海[1] 李湘秦[1] 吴勇[1] 

机构地区：[1]深圳市公安局刑事科学技术研究所,广东深圳518040

出　　处：《刑事技术》2014年第6期42-43,46,共3页Forensic Science and Technology

摘　　要：目的建立混合斑中精子DNA批量自动提取的方法。方法对56例混合斑经第一步消化后的精子沉淀用DNA IQ试剂盒和Biomek2000全自动核酸工作站自动提取纯化,3130xl型遗传分析仪分析结果。结果56例混合斑检材均得到较好的STR分型结果。结论 DNA IQ试剂盒和Biomek2000全自动核酸工作站批量自动提取纯化混合斑的方法快速简便,可应用于法庭科学实践。Objective To establish a method of automatic extraction of sperms DNA from mixed stain. Methods 56 mixed stain samples were collected from casework. The samples were extracted by using DNA IOTM System and Biomel2000 Laboratory Automation Workstation, and then analyzed with 3130xl genetic analyzer. Results STR genetic typing was obtained from all 56 mixed stain samples. Conclusions DNA IQTM System and Biomek2000 Laboratory Automation Workstation can be used for sperms DNA extraction from mixed stain in forensic casework.

关 键 词：混合斑 DNA IQTM自动化磁珠法 

分 类 号：Q523[生物学—生物化学]