不同年龄反复自然流产患者封闭抗体检测结果分析  

Analysis test results of blocking antibody in different age patients with recurrent spontaneous abortion

作　　者：莫扬[1] 郝世勇[1] 朱宇芳[1] 杨燕[1] 

机构地区：[1]湖北省襄阳市中心医院检验科,湖北襄阳441021

出　　处：《临床和实验医学杂志》2014年第23期1987-1990,共4页Journal of Clinical and Experimental Medicine

摘　　要：目的采用荧光标记流式细胞分析技术,研究封闭抗体与反复自然流产(RSA)发病的关系,探讨不同年龄RSA患者中封闭抗体阴性率的差异。方法 239例RSA患者按年龄分组分析CD3、CD4、CD8淋巴细胞比率,计算封闭效率(BE),统计封闭抗体阴性率,并对其中35例封闭抗体阴性者进行主动免疫治疗,观察和记录免疫治疗前后患者封闭抗体的变化。结果 239例反复自然流产患者共检出封闭抗体阴性者150例,阴性率62.7%;按年龄分组其淋巴细胞比率、封闭效率、封闭抗体阴性率的年龄差异无统计学意义;35例接受主动免疫治疗的患者中,11例患者BA全部转为阳性,14例抗CD-BE水平有所提高,治疗有效率71.4%。结论封闭抗体是妊娠的产物,与患者年龄因素无关;封闭抗体检测对评估反复自然流产有一定价值,淋巴细胞主动免疫治疗有助于封闭抗体转阳。Objective To investigate the relationship between the blocking antibody（ BA）and the recurrent spontaneous abortion（ RSA） by flow cytometry analysis technology,and to explore the difference of BA negative rate in different age′s patients. Methods The CD3,CD4, CD8 lymphocytes percentage in 239 cases of RSA patients was analyzed by age group. Computing blocking efficiency,statistical treatment the negative rate of BA,and among 35 cases of BA negative for initiative lymphocyte immunotherapy. To observe and record the change of BA in patients before and after the treatments. Results 150 cases of BA negative were detected from 239 RSA patients. The negative rate of BA was 62. 7﹪. According to the age,the lymphocytes percentage,BE,negative rate of BA had no significant statistic difference. Among the 35 BA negative reactors,there were 11 changing positive,14 cases of CD-BE improved after the lymphocyte immunotherapy,and the response rate was 71. 4﹪. Conclusion BA is the outcome of pregnancy. There is no relationship with age of patients. The detection of BA is valuable for evaluating RSA, and the lymphocyte immunotherapy could help BA to change positive rate.

关 键 词：反复自然流产 封闭抗体 主动免疫 

分 类 号：R714.21[医药卫生—妇产科学]