康脑液对局灶性脑缺血再灌注大鼠的脑保护作用机制研究  

Study mechanism of protective effects of Kangnao liquid on brain of rats with focal cerebral ischemic-reperfusion injury

作　　者：肖志娟[1] 赵志敏[1] 邹玉安[2] 薛茜[2] 王静[3] 

机构地区：[1]河北北方学院研究生部,河北张家口075000 [2]河北北方学院附属第一医院神经内科,河北张家口075000 [3]河北北方学院生命科学研究中心,河北张家口075000

出　　处：《中国临床药理学杂志》2014年第12期1101-1104,共4页The Chinese Journal of Clinical Pharmacology

基　　金：河北省卫生厅课题基金资助项目(20090591)

摘　　要：目的观察康脑液对SD大鼠脑缺血再灌注后血管新生和碱性成纤维细胞生长因子(b FGF)、磷脂酰肌醇3激酶/蛋白激酶B(Pi3k/akt)表达的影响。方法雄性SD大鼠随机分为6组:3个剂量康脑液组[24,12,6g·(kg·d)-1]、尼莫地平组[1 mg·(kg·d)-1]、模型组及假手术组。栓线制备大鼠右侧大脑中动脉闭塞(MCAO)模型。于缺血2 h再灌注12,24,48,72,168 h,用免疫组化法观察b FGF、Pi3k及akt的表达。再灌注72 h用免疫组化法观察血管性血友病因子(v WF)的表达,计算微血管密度(MVD)。结果与模型组相比,康脑液组b FGF、Pi3k及akt表达明显增多,脑组织血管显著增多(P<0.05或P<0.01)。结论康脑液通过增加b FGF、Pi3k/akt的表达从而促进缺血区血管新生及减少缺血区神经功能损害,起到脑保护作用。Objective To observe the effect of Kangnao liquid （ KNY） on the expression of basic fibroblast growth factor （ bFGF ） , phosphatidyli-nositol 3-kinase/protein kinase B （ Pi3k/akt） and microvascular densi-ty （ MVD） in rats with focal cerebral ischemia -reperfusion.Methods The male SD rats were randomly divided into six groups：three dose KNY groups [ 24 , 12 , 6 g . （ kg . d ） -1 ] , nimodipine group [1 mg. （kg. d） -1], model group, sham group.The animal models of middle cerebral artery occlusion （ MCAO） were established by a monofi-lament method from right internal carotid artery.Immunohistochemical method was used to investigate the expression of bFGF and Pi 3 k/akt in rats on 12,24,48,72,168 h of reperfution injury after ischemia for 2 h and to mark neovascular vWF after 72 h of reperfution and to calculate MVD.Results Compared with model group , the expression of bFGF , Pi3k/akt at different time points and MVD of brain tissue were signifi-cantly increased of KNY groups （ P〈0.05 or P〈0.01 ）.Conclusion KNY can increase the expression of bFGF and Pi 3 k/akt , promote tissue angiogenesis , then protect nerve cells of cerebral infarction areas.

关 键 词：康脑液 缺血再灌注 微血管密度 碱性成纤维细胞生长因子 磷脂酰肌醇-3-羟激酶/蛋白激酶B通路 

分 类 号：R285.5[医药卫生—中药学] R971[医药卫生—中医学]