2-十二烯酸对人肝癌细胞毒性作用研究  

Toxic effects of 2-dodecenoic acid on human hepatoma cells

作　　者：俞发荣[1] 连秀珍[1] 谢明仁[1] 李作平[1] 李登楼[1] 

机构地区：[1]甘肃政法学院甘肃省证据科学技术研究与应用重点实验室,兰州730070

出　　处：《中国临床药理学杂志》2014年第12期1108-1110,1123,共4页The Chinese Journal of Clinical Pharmacology

基　　金：甘肃省基础研究创新群体基金资助项目(145RJIA333);兰州市科技发展计划基金资助项目(2010-1-128)

摘　　要：目的探讨2-十二烯酸对人肝癌细胞的毒性作用。方法分别给予人肝癌细胞3个浓度(0.1,0.3,0.9 mg·L-1)2-十二烯酸,培养6,12,18,24,48 h,用台盼蓝法、MTT法、流式细胞仪检测2-十二烯酸对人肿瘤细胞的毒性作用。结果培养48 h,3个浓度的2-十二烯酸对人肝癌细胞增殖抑制率分别为45.46%,7.16%,9.18%,与对照组比差异有统计学意义(P﹤0.01);凋亡率分别为14.8%,20.2%,24.2%。结论 2-十二烯酸对人肝癌细胞增殖有明显的毒性作用。Objective To investigate the toxicity of 2-dodecenoic acid on human hepatocarcinoma cells.Methods The present study is to in-vestigate the toxic effects of 2 -dodecenoic acid on human hepatoma tumor cells by using trypan blue staining , MTT assay , and flow cytometry analysis.Results After cancer cells were treated with concentrations of 0.1 , 0.3 ,0.9 mg. L-1 2-dodecenoic acid at 6 h, 12 h, 18 h, 24 h, and 48 h, respectively.Compared to the control group , 48 h after treat-ment, the inhibition rates of the hepatoma cells proliferation in treatment group were increased by 45.46%, 67.16%, 79.18%at the above three concentrations , respectively （ P〈0.01 ）.Flow cytometry analysis showed that the apoptosis rates after the 2-dodecenoic acid treatment were in-creased by 14.8%, 20.2% and 24.2% over the control group.Conclusion These results indicated that the 2-dodecenoic acid has a significant toxic effect on human hepatoma cells.

关 键 词：2-十二烯酸 人肝癌细胞 毒性作用 细胞周期 凋亡 

分 类 号：R965.1[医药卫生—药理学] R979.1[医药卫生—药学]