大鼠被动皮肤过敏性实验中最佳佐剂的选择研究  

Study on the best choice of adjuvant on passive cutaneous anaphylaxis in rats

作　　者：荆宝琴[1] 张金晓[1] 王磊[1] 段蕊[1] 张宗鹏[1] 

机构地区：[1]天津药物研究院天津市新药安全评价研究中心,天津300193

出　　处：《中国临床药理学杂志》2014年第12期1114-1116,共3页The Chinese Journal of Clinical Pharmacology

基　　金：国家科技重大专项基金资助项目(2013ZX09302301);天津创新药物安全评价技术平台基金资助项目

摘　　要：目的比较研究是否添加佐剂及加入不同佐剂对大鼠被动皮肤过敏性实验(PCA)的影响。方法以卵清白蛋白为致敏原,SD大鼠随机分为4组:以不加佐剂为空白组,3个不同佐剂(百日咳菌液0.25 m L/只、氢氧化铝0.5 m L/只、百白破联合疫苗0.25 m L/只)实验组,进行PCA实验,判断各组的过敏反应情况。结果各组均能引起不同程度的过敏反应,佐剂组过敏反应发生率和严重程度都明显高于空白组,过敏反应从大到小依次为百白破联合疫苗>百日咳菌液>氢氧化铝;雌性大鼠发生过敏反应的动物数目及反应程度均高于雄性大鼠。结论不同佐剂对大鼠被动皮肤过敏性实验的影响有差异,综合考虑不同因素,氢氧化铝可作为被动皮肤过敏性实验中佐剂的最佳选择。Objective To get the best choice of adjuvant by researching the influence of the adjuvant whether it is added or not on passive cutane-ous anaphylaxis test and the distinction of different adjuvant which is used on passive cutaneous anaphylaxis （ PCA） test.Methods With ovalbu-min （OVA） as allergens, SD rats were injected three different adjuvant , pertussis bacteria liquid （ 0.25 mL per animal ） , aluminium hydroxide （ 0.5 mL per animal ） , diphtheria-pertussis-tetanus combined vaccine （ DPT） （ 0.25 mL per animal ） on PCA test.The skin allergic reaction was investigated.Result Each group can cause allergic reaction with different degrees.The incidence of allergic reaction in adjuvant group was obviously higher than blank group.According to the incidence and severity of allergic reaction , the result from large to small is DPT vac-cine , pertussis bacteria liquid , aluminium hydroxide.The number of ani-mals with allergic reaction in female rats is higher than the number in male rats.Conclusion Allergic reaction using different adjuvant on PCA test was different .Taking comprehensive consideration of different adjuvant in different factors , such as purchase , transportation , price and the sensitization effect , aluminium hydroxide can be the best choice on PCA test.

关 键 词：被动皮肤过敏反应 佐剂 氢氧化铝 百日咳菌液 百白破联合疫苗 

分 类 号：R977.29[医药卫生—药品] R965.1[医药卫生—药学]