清热化瘀滋阴方药效学研究和对狼疮小鼠肾组织凋亡蛋白的影响  

Pharmacodynamic study of Qingrehuayuziyin prescription and its effect on expression of apoptosis protein on renal tissue in lupus mice

作　　者：梁卫[1] 梁涛[2] 陆瑜[1] 张丽玲[1] 陈晔[1] 

机构地区：[1]解放军第454医院中医科,南京210002 [2]南京中医药大学,南京210023

出　　处：《中国临床药理学杂志》2014年第12期1120-1123,共4页The Chinese Journal of Clinical Pharmacology

基　　金：南京军区科技创新课题基金资助项目(10MA054)

摘　　要：目的观察清热化瘀滋阴方的抗炎镇痛作用和对狼疮性MRL/lpr小鼠肾组织凋亡蛋白的影响。方法制备小鼠醋酸扭体和二甲苯致小鼠耳肿胀模型,动物分为5组,模型组、3个剂量(12,24,48 g·kg-1)清热化瘀滋阴方组及阿司匹林组(300 mg·kg-1),灌胃3 d,观察给药前后镇痛抗炎作用。制备大鼠Ⅲ型变态反应模型,动物分为5组,模型组、3个剂量(6,12,24 g·kg-1)清热化瘀滋阴方及泼尼松组(5 mg·kg-1),灌胃10 d,观察给药后大鼠皮肤反应程度。MRL/lpr小鼠分为4组,模型组、清热化瘀滋阴方组(24 g·kg-1)、泼尼松组(10mg·kg-1)及清热化瘀滋阴方联合泼尼松组(24 g·kg-1+10 mg·kg-1),灌胃4周,检测对肾组织Fas、Fas L凋亡蛋白的表达。结果清热化瘀滋阴方能够明显减少扭体次数和耳廓肿胀度;抑制大鼠Ⅲ型变态反应皮肤反应程度;同时显著升高MRL/lpr小鼠肾组织Fas、Fas L蛋白表达。结论清热化瘀滋阴方具有抗炎镇痛、抑制变态反应和促进肾组织细胞凋亡作用。Objective To investigate the effects of Qingrehuayuziyin （ QRHYZY） prescription on anti -inflammatory and analgesic , and its effect on the expression of apoptosis protein on renal tissue in the MRL /lpr mice.Methods The acetic acid writhing test was applied to observe the analgesic effect of QRHYZY prescription in mice .The xylene was used to induce the ear swelling in mice .Mice were divided into 5 groups：model group,QRHYZY prescription group（6,12,24 g. kg -1 ） ,aspirin group（300 mg. kg -1 ）.The medicine was given by intragastric adminis-tration for 3 days.The variety of inflammatory and analgesic after QRHYZY prescription was observed.The degree of skin response were measured in Ⅲallergic reaction model.Rats were divided into 5 groups：model group ,QRHYZY prescription group（6,12,24 g. kg -1）,predi-nisone group（5 mg. kg -1）.The medicine was given by intragastric ad-ministration for 10 days.MRL/lpr mice were divided into 4 groups：model group,QRHYZY prescription group （24 g. kg-1）,predinisone group（10 mg. kg -1）,QRHYZY prescription＋predinisone group（24 g. kg -1 ＋10 mg. kg -1 ）.Intervention therapy was applied to MRL/lpr mice for 4 weeks in order to detect Fas and FasL protein expression on renal tissue.Results QRHYZY prescription reduced the writhing times in mice＆amp;nbsp;caused by the acetic and ear swelling caused by the xylene .The degree of skin response were reduced by QRHYZY prescription in Ⅲ allergic reaction.The expression of Fas and FasL protein were significantly increased by QRHYZY prescription.Conclusion The QRHYZY prescription is good at anti -inflammation ,analgesia.TheⅢallergic reaction was inhibited obviously and can promote lupus renal tissue cell apoptosis.

关 键 词：清热化瘀滋阴方 药效 耳肿胀 变态反应 凋亡蛋白 

分 类 号：R285.5[医药卫生—中药学] R976[医药卫生—中医学]