LC-MS/MS测定人血浆中阿罗洛尔浓度  

Determination of Arotinolol Concentration in Human Plasma by LC-MS/MS

作　　者：李力[1] 肖昌钱[1] 朱逢佳[1] 廉洪[1] 韩奇[1] 刘炜[1] 

机构地区：[1]浙江医院药剂科,杭州310013

出　　处：《中国现代应用药学》2014年第12期1496-1499,共4页Chinese Journal of Modern Applied Pharmacy

基　　金："重大新药创制"科技重大专项(2013ZX09303005);国家自然科学基金(31201040)

摘　　要：目的 建立LC-MS/MS方法测定人血浆中阿罗洛尔的浓度。方法 人血浆样品用乙酸乙酯提取后,选用ZORBAX Extend C18（2.1 mm×100 mm,3.5-m）色谱柱,以乙腈-水（80∶20,含0.1%甲酸）为流动相,流速为0.30 m L·min^-1,采用电喷雾离子化源,正离子方式,多反应监测（MRM）扫描方式进行监测,用于定量分析的离子反应分别为m/z372.3→315.9（阿罗洛尔）和m/z 267.2→145.0（内标阿替洛尔）。结果 血浆中阿罗洛尔的线性范围为0.5~1 000 ng·m L^-1（r=0.995 2）,定量下限为0.5 ng·m L^-1;日内、日间RSD均〈15%;低、中、高3个浓度的提取回收率分别为（80.6±1.6）%,（83.2±3.1）%和（87.5±4.5）%。结论 该方法快速、灵敏、准确,专属性强,重复性好,适用于人血浆中阿罗洛尔浓度的测定,可应用于阿罗洛尔的血药浓度检测和药动学研究。OBJECTIVE To develop a LC-MS/MS method for determination of arotinolol concentration in human-plasma. METHODS The plasma samples were processed with ethyl acetate extraction and separated on ZORBAX Extend C18 column（2.1 mm×100 mm, 3.5 μm） and eluted with acetonitrile-water（80∶20, containing 0.1% formic acid）. Detection of the analyte was achieved using positive ion electrospray ionization（ESI） in the multiple reaction monitoring（MRM） mode. The MS/MS ion transitions monitored were m/z 372.3→m/z 315.9 and m/z 267.2→m/z 145.0 for arotinolol and internal standard, respectively. RESULTS The linear calibration curve of arotinolol was obtained concentration range of 0.5-1 000 ng·m L^-1（r=0.995 2）, with the lower limit of quantitation of 0.5 ng·m L^-1. Intra-day and inter-day relative standard deviations were both 15%. The recoveries of low, middle and high concentrations were（80.6±1.6）%,（83.2±3.1）% and（87.5±4.5）%, respectively. CONCLUSION The established method is rapid, sensitive, accurate, specific and reliable, and suitable for the determination of arotinolol in human plasma and pharmacokinetic study.

关 键 词：阿罗洛尔 液质联用 血药浓度 药动学 

分 类 号：R917.101[医药卫生—药物分析学]