初筛Rh(D)阴性无缘献血者弱D和Del表型调查  

Investigation on the distribution of weak D and Del phenotype in unrelated blood donors with negative Rh(D)

作　　者：张松[1] 常利[2] 宋任浩[3] 

机构地区：[1]河北医科大学第三医院检验科,石家庄市050051 [2]河北医科大学第三医院康复科,石家庄市050051 [3]河北省血液中心,130100

出　　处：《实用医学杂志》2014年第24期4028-4030,共3页The Journal of Practical Medicine

基　　金：河北省医学科学研究重点课题计划(编号:20130133)

摘　　要：目的:了解Rh(D)阴性献血者中弱D和Del表型的分布情况。方法:对初筛Rh(D)阴性无亲缘关系献血者采用间接抗球蛋白试验检测弱D型,采用吸收放散法检测Del型。结果:在409例盐水法初筛为Rh(D)阴性无亲缘关系献血者中,检出弱D表型27例,占6.61%,Del表型61例,占14.91%,确证Rh(D)阴性321例,占78.48%。在确证Rh(D)阴性献血者中ccee占49.14%,其次是Ccee,占23.47%;弱D表型中cc Ee占4.16%,其次是Ccee,占1.71%;Del表型中Ccee占10.02%,CCee占1.71%。结论:对初筛Rh(D)阴性的献血者应采用更为敏感的试验确证是否为弱D表型或Del表型。为保障输血安全,弱D表型和Del表型献血者应作Rh D阳性看待,而作为受血者则应视为Rh D阴性。Objective To investigate the distribution of weak D and Del phenotype in unrelated blood donors with negative Rh （D）. Methods Four hundred and nineRhD （-） unrelated donors were screened by the saline agglutination method. Type weak D was detected by the indirect antiglobulin test, and type Del was detected by absorb radiation method. Results In 409 RhD （-） samples, 27 cases （6.61%） of type weak D were checked out, and 61 cases （14.91%） were type Del and 321 cases （78.48%） were corroborate for RhD （-）, In the confirmed RhD （-） blood donors, phenotype ccee was most （49.14 %）, followed by Ccee （23.47%）, People with phenotype ccEe accounted for 4.16% in type weak D, followed by Ccee （1.71%）. Ccee accounted for 10.02% in type Del, and Ccee accounted for 1.71%. Conclusion RhD （-） donors screened by regular testing should adopt a more sensitive test for verifing type weak D or Del. In order to ensure the security of blood transfusion, people with type weak D and Del should be regarded as RhD positive blood donors, and the RhD negative people deemed to be recipients.

关 键 词：初筛 RH(D)阴性 弱D表型 DEL表型 

分 类 号：R446.6[医药卫生—诊断学]