不同前处理方法对鲜葡萄枝蔓中茋类化合物的影响研究  

Influence of different pretreatment methods on stilbenoids in grapevine

作　　者：谢红旗[1,2] 杨星星[1,2] 曾建国[1,3,2] 

机构地区：[1]湖南农业大学园艺园林学院,植物功能成分利用2011协同创新中心,湖南长沙410128 [2]湖南农业大学国家中药材生产(湖南)技术中心,湖南长沙410128 [3]湖南省中药提取工程研究中心,湖南长沙410331

出　　处：《中草药》2014年第24期3553-3558,共6页Chinese Traditional and Herbal Drugs

基　　金：国家科技支撑计划资助项目(2012BAI29B04);国际合作项目(2013DFA31790)

摘　　要：目的研究不同前处理方法对鲜葡萄Vitis vinifera枝蔓茋类化合物的影响。方法采用HPLC法同时测定葡萄枝蔓中活性成分白藜芦醇和ε-葡萄素,对新剪枝的葡萄枝蔓进行不同方式的干燥处理:阴干、烘干、冷藏;对葡萄枝蔓采取整枝、切片和粉碎3种方式处理,考察干燥过程中葡萄枝蔓中白藜芦醇和ε-葡萄素的动态变化规律。结果新鲜葡萄枝蔓中白藜芦醇和ε-葡萄素的量均较低,采用整枝阴干的前处理方式,白藜芦醇随着阴干时间的延长而发生显著变化,由初始的0.063 mg/g上升至7.175 mg/g,ε-葡萄素也由初始的0.838 mg/g上升至4.225 mg/g;二者均随时间延长达到一定程度后又呈现下降的趋势。而对于采用将葡萄枝蔓进行切片阴干、切片冷藏、粉碎阴干、粉碎冷藏和不同温度快速烘干处理,白藜芦醇和ε-葡萄素变化不明显。结论不同的处理方式能影响到剪枝后葡萄枝蔓中的白藜芦醇和ε-葡萄素量的变化。采取整枝阴干处理,白藜芦醇和ε-葡萄素随时间的延长均呈现先显著升高再下降的变化趋势,白藜芦醇和ε-葡萄素在一定阶段能达到可开发利用的水平。Objective To study the influence of different pretreatment methods of grapevine on the content of stibenoids in grapevine. Methods The concentration of resveratrol and ε-viniferin was determined by HPLC, the fresh grapevine sample after picked was pretreated by different methods such as drying in the shade, drying in a dryer under different temperature, keeping in cold storage, slicing, pulverization, or whole treating, and the dynamic changes of stilbenoids in grapevine was investigated. Results The contents of resveratrol and ε-viniferin in the fresh sample were very low, but the contents of resveratrol and ε-viniferin in the grapevine after picked would change significantly if treated as drying in the shade with whole branch, especially the content of resveratrol would rise from 0.063 mg/g to 7.175 mg/g with the extension of treating time, and the content of ε-viniferin would rise from 0.838 mg/g to 4.225 mg/g, but at some extent, the content would decline. The contents of resveratrol and ε-viniferin would not rise if the sample was pretreated as drying in the shade after slicing, drying in a dryer or keeping in cold storage. Conclusion The influence of different pretreatment methods to the contents of resveratrol and ε-viniferin is very large, during drying process in the shade with whole branch, the contents of resveratrol and ε-viniferin change obviously and display the tendency of increasing at the earlier phase of the drying process, and then decrease with the extension of time. The contents of resveratrol and ε-viniferin can reach the level of utilization with the pretreatment of drying process in the shade.

关 键 词：葡萄枝蔓 茋类化合物 白藜芦醇 ε-葡萄素 前处理方法 

分 类 号：R282.23[医药卫生—中药学]