HPLC-DAD法同时测定八味沉香散中的6种成分  

Simultaneous determination of six components in Bawei Chenxiang Powder by HPLC-DAD

作　　者：吴剑涓[1] 李文明[2] 李腾飞[2] 

机构地区：[1]天津市环湖医院药剂科,天津300060 [2]天津医科大学药学院,天津300070

出　　处：《中草药》2014年第24期3569-3572,共4页Chinese Traditional and Herbal Drugs

摘　　要：目的建立同时测定八味沉香散中没食子酸、原儿茶酸、木香烃内酯、去氢木香内酯、去氢二异丁香酚、11-羰基-β-乙酰乳香酸6种成分的HPLC-DAD方法。方法采用RP-HPLC法,Waters XBridge-C18色谱柱（250 mm×4.6 mm,5.0μm）,甲醇-乙腈（50∶50,A）和甲醇-0.1%乙酸水溶液（10∶90,B）为流动相,体积流量0.8 m L/min,梯度洗脱;柱温35℃;进样量10μL。分别对线性关系、精密度、重复性、稳定性及加样回收率进行考察。结果被测定的6种指标成分没食子酸在1.5~30.0 mg/L（r=0.999 0）、原儿茶酸在1.0~20.0 mg/L（r=0.999 2）、木香烃内酯在2.0~40.0 mg/L（r=0.999 1）、去氢木香内酯在2.0~40.0 mg/L（r=0.999 3）、去氢二异丁香酚在0.2~4.0 mg/L（r=0.999 4）、11-羰基-β-乙酰乳香酸在3.5~70.0 mg/L（r=0.999 1）线性关系良好;精密度、重复性良好,RSD均小于2.0%;在室温条件下24 h内稳定;平均加样回收率在98.49%~101.55%（RSD≤2.0%）。结论该方法简便、准确,重复性好,为八味沉香散的质量控制提供实验依据。Objective To develop an HPLC method for simultaneous determination of gallic acid, protocatechuic acid, costunolide, dehvdrocostuslactone, ehydrodiisoeugenol, and acetyl-11-keto-β-boswellic acid in Bawei Chenxiang Powder（BCP）. Methods The chromatographic separation was achieved on a Waters XBridge-C18 column（250 mm × 4.6 mm, 5.0 μm） with methanol-acetonitrile（50∶50, A） and methanol-0.1% acetic acid（10∶90, B） as mobile phases at the flow rate of 0.8 m L/min for gradient elution, and the column temperature was 35 ℃. Its linear relationship, precision, repeatability, stability, and recoveries were investigated. Results The seven components were well separated and showed good linearity, such as gallic acid 1.5—30.0 mg/L（r = 0.999 0）, protocatechuic acid 1.0—20.0 mg/L（r = 0.999 2）, costunolide 2.0—40.0 mg/L（r = 0.999 1）, dehvdrocostuslactone 2.0—40.0 mg/L（r = 0.999 3）, ehydrodiisoeugenol 0.2—4.0 mg/L（r = 0.999 4）, and acetyl-11-keto-β-boswellic acid 3.5—70.0 mg/L（r = 0.999 1）. The precision was good, and RSD value was less than 2.0%. The repeatability was good, and RSD was less than 2.0%. The stability was good in 24 h. The average recoveries were between 98.49% and 101.55%（RSD ≤ 2.0%）. Conclusion The method is accurate, sensitive, credible, and repeatable. It can be applied to the quality control of BCP.

关 键 词：八味沉香散 HPLC 没食子酸 原儿茶酸 木香烃内酯 去氢木香内酯 去氢二异丁香酚 11-羰基-Β-乙酰乳香酸 

分 类 号：R286.02[医药卫生—中药学]