过山蕨总黄酮抗肺癌作用及其对赖氨酰氧化酶抑制活性  

Anti-lung cancer effect of total flavonoids from Camptosorus sibiricus and their inhibition on lysyl oxidase

作　　者：刘迎辉[1] 戚笑笑 李红[1] 刘霖鑫 侯西栋[1] 李刚刚[1] 王沛[1] 杜钢军[1] 

机构地区：[1]河南大学药学院药物研究所,河南开封475004

出　　处：《中草药》2014年第24期3573-3578,共6页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81173094);河南省高校青年骨干教师项目(2010GGJS-025)

摘　　要：目的观察过山蕨总黄酮对小鼠肺癌的干预作用,研究其可能的抗肿瘤机制。方法乌拉坦致小鼠肺癌模型观察过山蕨总黄酮(30、100 mg/kg)对小鼠Lewis肺癌的预防作用,小鼠Lewis肺癌被动转移模型考察过山蕨总黄酮(30、100 mg/kg)对小鼠肺癌转移的影响,小鼠Lewis肺癌复发模型验证过山蕨总黄酮(30、100 mg/kg)对小鼠肺癌的机体微环境清道夫作用,Western blotting和免疫组化染色法考察过山蕨总黄酮(30、100 mg/kg)对肺癌组织赖氨酰氧化酶(LOX)的影响,通过检测毛细血管通透性考察过山蕨总黄酮对肺癌组织的血管正常化作用。结果过山蕨总黄酮高、低剂量(100、30 mg/kg)均能显著降低乌拉坦诱导的小鼠肺癌发生率,明显减少小鼠Lewis肺癌被动转移率和小鼠Lewis肺癌皮下肿瘤切除后复发率。过山蕨总黄酮高、低剂量均能显著下调小鼠肺癌组织LOX表达,阻止肿瘤切除小鼠血清脂质过氧化物形成,促进肺癌组织血管正常化。结论过山蕨总黄酮可能通过抑制LOX表达实现对肺癌的干预作用。Objective To observe the intervention of total flavonoids from Camptosorus sibiricus（TFCS） on lung cancer in mice and to study its possible antitumor mechanism. Methods The lung cancer model induced by urethane was used to investigate the preventive action of TFCS on lung cancer, the passive pulmonary metastatic model of Lewis lung cancer was used to evaluate the effect of TFCS on tumor metastasis, the scavenger effect of TFCS on system microenvironment was observed in the recurrent tumor model of Lewis lung cancer, the effect of TFCS on lysyl oxidase（LOX） was investigated by Western blotting and immunohistochemical staining, and the vascular normalization function of TFCS was examined by capillary permeability in carcinogenetic lung tissues. Results TFCS（30 and 100 mg/kg） could decrease the lung carcinogenesis induced by urethane and reduce the passive pulmonary metastasis and recurrence after tumor removal in Lewis lung cancer-bearing mice. TFCS（30 and 100 mg/kg） could also downregulate the expression of LOX in lung cancer tissue, prevent serum lipid peroxide formation in mice with tumor removal, and promote vascular normalization of lung cancer tissue. Conclusion TFCS as an LOX inhibitor has a definite preventive action on lung cancer.

关 键 词：过山蕨总黄酮 肺癌 赖氨酰氧化酶 血管正常化 肺癌转移 

分 类 号：R286.91[医药卫生—中药学]