参附汤有效组分配伍对离体大鼠心肌细胞L型钙通道的影响  

Regulation on in vitro myocardial cell L type calcium channel of rats with active component compatibility of Shenfu Decoction

作　　者：杨勇[1] 任长虹[2] 孟红旭[3] 吴晓丹[1] 

机构地区：[1]北京中医药大学方药系,北京100029 [2]首都医科大学宣武医院低氧医学研究室,北京100053 [3]中国中医科学院西苑医院,北京100091

出　　处：《中草药》2014年第24期3594-3598,共5页Chinese Traditional and Herbal Drugs

基　　金：教育部中央高校基金项目(JYBZZ-JS005);教育部留学回国人员科研启动基金

摘　　要：目的观察参附汤有效组分及其配伍对大鼠心室肌细胞L型钙通道电流的作用。方法采用Langendorff心脏灌流,急性酶解法获得单个心室肌细胞,全细胞膜片钳技术记录并分析参附汤人参有效组分人参皂苷Re、Rg1、Rb1和附子有效组分乌头原碱及其配伍对心肌细胞L型钙通道电流的影响。结果人参皂苷Re和Rg1能够可逆性地阻断L型钙通道电流,给予不同浓度人参皂苷Re(3、10、30、100μmol/L)和Rg1(10、100μmol/L)后L型钙通道电流幅度呈现随药物浓度增加逐渐减小的趋势;人参皂苷Rb1(10、100μmol/L)对L型钙通道电流幅度变化无明显影响;乌头原碱能够不可逆地增强L型钙通道电流,给予不同浓度乌头原碱(10、100μmol/L)后L型钙通道电流幅度随药物浓度增加逐渐增大。联合使用人参皂苷Re(20μmol/L)和Rg1(80μmol/L)对L型钙通道的抑制作用强于单独使用人参皂苷Re和Rg1;而人参皂苷Rg1(80μmol/L)、Re(20μmol/L)和乌头原碱(100μmol/L)联合应用对人参皂苷Rg1和Re配伍对L型钙通道电流的抑制作用无明显影响。结论参附汤有效组分人参皂苷Rg1、Re抑制L型钙通道电流,而乌头原碱对L型钙通道电流有不可逆的增强作用,人参皂苷Rg1、Re配伍对L型钙通道电流的抑制作用增强,乌头原碱配伍后对人参皂苷Rg1、Re的L型钙通道电流抑制作用无明显影响。Objective To investigate the regulation of in vitro myocardial cell L type calcium channel of rats with active component of Shenfu Decoction and its combination. Methods With Langendorff cardiac infusion, the single ventricular myocardial cell with acute enzymylosis approach was acquired and the voltage current of L type calcium channel was recorded with whole cell patch technique. Results The active ginsenosides Re（3, 10, 30, and 100 μmol/L）, and Rg1（10 and 100 μmol/L） can reversibly reduce L type calcium current in a dose dependant manner, Rb1（10 and 100 μmol/L） has no effect on L type calcium current; the active component aconine（10 and 100 μmol/L） of aconite could irreversibly enhance L type calcium current in a dose dependant manner; The combination of ginsenosides Re（20 μmol/L） and Rg1（80 μmol/L） also reduced the L type calcium current, which is more significant than either ginsenosides Rg1 and Re; The inhibitory rate of the combination of ginsenosides Rg1, Re and aconine（100 μmol/L） has no difference from the combination of ginsenosides Rg1 and Re. Conclusion The active component ginsenosides Rg1 and Re of Shenfu Decoction can reduce the L type calcium current, while aconine can irreversibly enhance the L type calcium current; The combination of ginsenosides Rg1 and Re can obviously increase the reduction of L type calcium current, while the combination of ginsenosides Rg1, Re and anonine can not change the reduction of L type calcium current.

关 键 词：参附汤 人参皂苷 乌头原碱 组分配伍 L型钙离子通道 

分 类 号：R285.5[医药卫生—中药学]