荧光猝灭法测定茶叶中的钐和钕  

Determination of Samarium(Ш)and Neodymium(Ш)in the Tea Based on Fluorescence Quenching Effect of Congo Red

作　　者：王勇[1] 彭焕军 邬小强 赵小辉[1] 贺薇[1] 杨琼[1] 

机构地区：[1]长江师范学院化学化工学院,重庆涪陵408000

出　　处：《光散射学报》2014年第4期411-414,共4页The Journal of Light Scattering

基　　金：国家自然科学基金项目(21175015);长江师范学院大学生创新创业训练计划(2012cxx112)

摘　　要：在pH为3.20-3.40的弱酸性介质中,稀土金属元素钐（Ш）与钕（Ш）均能使刚果红的荧光显著猝灭。实验表明：刚果红的最大发射波长（λem）为419nm,在钐（Ш）和钕（Ш）的存在下,刚果红荧光猝灭程度（ΔF）与钐（Ш）和钕（Ш）的浓度呈线性关系。钐（Ш）和钕（Ш）的检出限分别为0.0826#g/mL和0.1088#g/mL,线性范围为0.004-2.8mg/mL和0.004-1.6mg/mL。文中优化了反应条件的影响,据此发展了以刚果红为荧光探针的灵敏、简便、快速测定钐（Ш）与钕（Ш）的新方法。将方法用于茶叶中钐（Ш）与钕（Ш）含量的快速测定,结果满意。In pH 3.20-3.40 faintly acid media, the fluorescence intensity of congo red can be quenched by rare earth metal such as Samarium （Ш）and Neodymium （Ш）. The results showed.the wavelength of maximum emission of congo red was at 419 nm,there was a linear relation between the quenching degree of the fluorescence and the concentration of samarium （Ш）and neodymium（Ш）, and the detection limit were 0. 0826 μg/mL （Sin）and 0. 1088 μg/ mL（Nd）, the linear range were 0. 004-2.8 mg/mL（Sm）and 0. 004-1.6 mg/mL（Nd）, respectively. The optimum reaction conditions were discussed. Congo red was chose as spectral probe, and a new, sensitive, simple and rapid method for determining Samarium（Ш）and Neodymium（Ш） was developed. The method had been applied to determination Samarium （Ш）and Neodymium（Ш）in tea samples with satisfactory results.

关 键 词：荧光猝灭 钐 钕 刚果红 

分 类 号：O65[理学—分析化学]