H2O2胁迫下豌豆初生根及抗氧化酶系统对外源Ca^2＋的响应  

Responses of primary root and antioxidase system to exogenous Ca^(2+) in pea under H_2O_2 stress

作　　者：刘会杰[1] 李胜[1] 马绍英[1] 张品南[1] 时振振 杨晓明[2] 

机构地区：[1]甘肃省干旱生境作物学重点实验室甘肃农业大学生命科学技术学院,甘肃兰州730070 [2]甘肃省农业科学院作物研究所,甘肃兰州730070

出　　处：《草业学报》2014年第6期189-197,共9页Acta Prataculturae Sinica

基　　金：现代农业食用豆产业技术体系专项资金(CARS-09);国家自然科学基金(31160304)资助

摘　　要：以豌豆品种"陇豌一号"为材料,通过对豌豆种子萌发初期初生根生理特性的研究,探索提高豌豆初生根外源H2O2胁迫条件下抗性能力的途径。运用生理生化方法,测定H2O2胁迫下豌豆初生根在外源Ca2+处理后的弯曲率和根系活力,并对豌豆初生根内的丙二醛(MDA)含量、相对膜透性、超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性进行测定。结果显示,80mmol/L的H2O2处理下的豌豆初生根正常生长受到显著抑制,但是经过Ca2+处理后,根系生长抑制作用得到缓解,根系活力得以恢复。外施10mmol/L Ca2+初生根MDA值较CK1降低了37.32%,并显著地提高了POD,SOD,CAT和APX的活性,其值分别为51.946U/(mg·min),865.174U/g FW,1.9739mmol/(L·g·min)和2.569μmol/(L·g·min)。总之,外源施加Ca2+能够有效降低H2O2造成的氧化胁迫,缓解对初生根细胞膜的伤害,降低质膜透性,增强初生根系抗氧化酶活性,达到抵抗逆境胁迫的目的。In order to explore methodology for improving the resistance of pea primary roots to H2O2 stress,physiological characteristics of pea primary roots were studied using apea variety ＂Longwan 1＂treated with Ca^2＋under H2O2 stress.Several physiological indexes like the curvature rate and root activity of pea primary roots were measured using physiological and biochemical methods.Some other indexes like the relative membrane permeability of primary roots,the concentration of malondialdehyde（MDA）in primary roots,the activities of superoxide dismutase（SOD）,peroxidase（POD）,catalase（CAT）and ascorbate peroxidase（APX）enzymes were also measured.The normal growth of pea primary roots under 80mmol/L H2O2 stress was obviously inhibited.However,the inhibitory effect on root growth was reduced and the root activity was restored after treating with Ca^2＋.The MDA content decreased 37.32% compared to controls after treatment with 10mmol/L exogenous Ca^2＋.The activities of four enzymes including POD,SOD,CAT and APX were enhanced and reached 51.946U/（mg·min）,865.174U/g FW,1.9739mmol/（L·g·min）and 2.569μmol/（L·g·min）,respectively.In summary,Ca^2＋（10mmol/L）significantly alleviated the damage to pea primary root cell membranes caused by H2O2 stress,reduced membrane permeability and promoted the antioxidant capacity of pea primary roots for stress resistance.

关 键 词：豌豆 H2O2 初生根 外源CA^2+ 抗氧化物酶 

分 类 号：S643.3[农业科学—蔬菜学]