基于纳米磁珠放大的高灵敏度纳米光纤生化传感器  

Highly Sensitive Optical Nanofiber Bio-Chemical Sensor Based on Magnetic Nanoparticles Amplication

作　　者：代方[1,2] 李凯伟[1] 周文超[1] 张威[3] 余慕欣 柳常清[4] 吴一辉[1] 

机构地区：[1]中国科学院长春光学精密机械与物理研究所应用光学国家重点实验室,吉林长春130033 [2]中国科学院大学,北京100049 [3]中国科学院苏州生物医学工程技术研究所,江苏苏州215163 [4]长春理工大学机电工程学院,吉林长春130033

出　　处：《光学学报》2014年第12期33-39,共7页Acta Optica Sinica

基　　金：国家863计划(2012AA040503);国家自然科学基金重点基金(11034007);国家青年科学基金项目(61102023);吉林省科技发展计划项目(20120329);吉林省自然科学基金(20101502);中国科学院科研装备研制项目(yz201353)

摘　　要：为满足临床诊断对生化传感器灵敏度和特异性的要求,研究了纳米磁珠对纳米光纤光传输特性的影响,以及纳米磁珠作为标记物同时完成目标待测物提纯和高灵敏度检测的可能性。详细介绍了锥形纳米光纤传感器的制备过程、表面处理方法和纳米磁珠的处理方法,设计实验检测到了直径200-300nm的单个纳米磁珠,验证了纳米磁珠的强信号放大作用,并通过实验验证了磁珠同时用作分离提纯和灵敏度增强的方案在纳米光纤传感器上的可行性。In order to meet the requirements of high sensitivity and specificity of clinical diagnosis, the effects of magnetic nanoparticles （MNPs） on the transmission performance of optical nanofiber are studied, as well as the possibility of MNPs functioned as labels to enhance the sensitivity of bio-chemical detection and the purificaiton of the determinand. The fabrication process of tapered optical nanofiber sensors, functionalization of optical nanofiber biosensors and MNPs are presented, Experiments are designed, the amplication function of MNPs is verified, and single MNP with the diameter from 200 nm to 300 nm is successfully resolved by optical nanofiber biosensor. The results of experiments demonstrate that the project of using MNPs as separation, purification and enhancing of sensitivity, can work well on optical nanofibers.

关 键 词：传感器 纳米光纤 纳米磁珠 单纳米粒子检测 生化检测 

分 类 号：O439[机械工程—光学工程]