黄连素对实验性自身免疫性脑脊髓炎大鼠神经保护作用的实验研究  

Experimental study of berberine on the neuroprotective effect in spinal cord of rats with experimental autoimmune encephalomyelitis

作　　者：朱振国[1] 黄艳君[2] 张元涛[1] 李剑敏[3] 

机构地区：[1]温州医科大学附属第一医院神经内科,浙江温州325000 [2]温州医科大学附属第一医院妇产科,浙江温州325000 [3]温州医科大学附属第一医院病理科,浙江温州325000

出　　处：《中国临床药理学杂志》2015年第1期59-62,共4页The Chinese Journal of Clinical Pharmacology

基　　金：国家自然科学基金资助项目(81070960);温州市科技局基金资助项目(Y20140292)

摘　　要：目的探讨黄连素(Ber)对实验性自身免疫性脑脊髓炎(EAE)大鼠脊髓内基质金属蛋白酶-9(MMP-9)、细胞间黏附分子-1(ICAM-1)的影响。方法Lewis大鼠24只随机分为3组:正常组、模型组和实验组。用行为学观察发病情况,常规HE染色观察中枢神经系统的病理变化;用免疫组化法和实时聚合酶链反应(RT-PCR)法,检测大鼠脊髓MMP-9、ICAM-1的变化。结果与模型组比较,实验组神经症状和病理改变减轻,脊髓内MMP-9和ICAM-1的蛋白及mRNA的表达均明显下降(P<0.05)。结论黄连素对EAE大鼠有神经保护作用,其可能机制与下调MMP-9和ICAM-1的蛋白和基因水平表达有关。Objective To explore the effects of berberine（ Ber） on the levels of matrix metallopeptidase- 9（ MMP- 9）,intercellular cell adhesion molecule- 1（ ICAM- 1） in spinal cord of rats with experimental autoimmune encephalomyelitis.Methods Twenty four Lewis rats were randomly divided into three groups： normal group,model group,Ber group.Neurologic symptoms of Lewis rats were daily examined.Then tissues were stained with Hematoxylin- eosin.Expression levels of MMP- 9and ICAM- 1 in spinal cord were quantified by immunohistochemistry.The transcriptional levels of MMP- 9,ICAM- 1 in spinal cord were examined by real- time polymerase chain reaction（ PCR）.Results Compared with model group,neurological impairment and pathological change were alleviated in Ber group.Protein and mRNA expression of MMP- 9and ICAM- 1 were decreased significantly in the spinal cord in Ber group（ P〈0.01）.Conclusion Berberine could have an effect on prevention on EAE in Lewis rat,may be related to its ability to the down regulations the expression levels of MMP- 9 and ICAM- 1.

关 键 词：黄连素 实验性自身免疫性脑脊髓炎 基质金属蛋白酶-9 细胞间黏附分子-1 神经保护作用 

分 类 号：R978.1[医药卫生—药品] R965.1[医药卫生—药学]