蚯蚓冻干粉对高血脂小鼠羟甲基戊二酸单辅酶A还原酶基因的调节作用  

Regulation of Earthworm Lyophilized Powder on Hydroxymethylglutaryl CoA Reductase Expression in Hyperlipidemic Mice

作　　者：武金霞[1] 吴红月[1] 单彪[2] 张贺迎[3] 

机构地区：[1]河北大学生命科学学院,河北保定071002 [2]邢台市人民医院,河北邢台054031 [3]河北大学生物工程技术研究中心,河北保定071002

出　　处：《中国医药工业杂志》2015年第1期24-26,47,共4页Chinese Journal of Pharmaceuticals

基　　金：河北省生物工程重点学科项目

摘　　要：以高脂饲料饲喂昆明种小鼠构建高血脂小鼠模型,灌胃给予高、中、低剂量(0.4、0.2和0.1 g/kg)蚯蚓冻干粉,每天1次,同时饲喂高脂饲料,共饲养10周。提取小鼠肝脏总RNA,应用反转录基因扩增(RT-PCR)技术检测羟甲基戊二酸单辅酶A还原酶(HMG-CoAR)信使核糖核酸(mRNA)水平。结果表明,试验组小鼠HMG-CoAR mRNA相对含量显著低于高脂饲料组小鼠,提示蚯蚓冻干粉能够降低高血脂小鼠血浆胆固醇水平,可能与下调肝HMG-CoAR mRNA表达有关。Kunming mice were fed with high-level lipid feeds to establish the hyperlipidemic mouse models. The mice were treated with earthworm lyophilized powder at dosages of 0.4, 0.2 and 0.1 g/kg once a day, meanwhile they were fed with high-level lipid feeds for 10 weeks. The total RNA of liver were extracted, and the changes of liver hydroxymethylglutaryl CoA reductase （HMG-CoAR） gene expression were analyzed by RT-PCR. The results indicated that earthworm lyophitized powder down-regulated HMG-CoAR mRNA expression markedly, suggesting that the effect of earthworm lyophilized powder on reducing serum cholesterol in hyperlipidemic mice was probably involved in downregulating HMG-CoAR mRNA expression.

关 键 词：蚯蚓冻干粉 高血脂症 胆固醇代谢 羟甲基戊二酸单辅酶A还原酶 

分 类 号：Q78[生物学—分子生物学]