高温热裂解人参皂苷Rk_1和Rg_5的制备工艺优化  

Optimization of preparing technology of ginsenoside Rk_1and Rg_5 by high-temperature pyrolysis

作　　者：关大朋 王欢[1,2] 李伟[1,2] 刘文丛[1] 王英平[2] 王佳[1,2] 郑毅男[1] 

机构地区：[1]吉林农业大学中药材学院,吉林长春130118 [2]中国农业科学院特产研究所,吉林长春130112

出　　处：《上海中医药杂志》2015年第1期91-95,共5页Shanghai Journal of Traditional Chinese Medicine

基　　金：中国博士后面上项目(2012M520483);科技部国家科技支撑计划项目(2011BAI03B01);公益性行业(农业)科研专项(201303111);吉林省科学技术厅医药产业发展专项基金资助项目(YYZX201136)

摘　　要：目的研究高压酸解制备人参稀有皂苷Rk1和Rg5的最佳工艺。方法利用正交设计优化高压酸解制备人参茎叶总皂苷,以HPLC峰面积分析人参皂苷Rk1和Rg5的生成。结果高温酸解制备人参皂苷Rk1和Rg5的最佳工艺参数为:压力125 MPa,温度120℃,溶剂浓度乙醇50%,甲酸0.05%,时间为2 h。结论优选人参稀有皂苷Rk1和Rg5的制备工艺合理可行。Objective To study the optimum conditions of prepares rare ginsenoside Rk1 and Rg5 by high temperature and high pressure technique （HTHPT）. Methods HTHPT was used to prepare rare ginsenoside from P. ginseng. The optimum extracting process was selected by the orthogonal test. The rare ginsenoside Rk1 and Rg5 were determined by HPLC. Results The optimum condition of HTHFT was as follows： the pressure was 125 MPa, the temperature was 120℃ the solvent was 50% ethanol, formic acid concentration was 0.05%, reaction time was 2h. Conclusion This preparation technology is valid and feasible.

关 键 词：人参皂苷 人参皂苷Rk1 人参皂苷Rg5 正交设计 LC-MS分析 

分 类 号：R283.6[医药卫生—中药学]