内毒素对大鼠小肠黏膜组织的影响及多价阳离子A的保护效应  

Influence of Endotoxin on Small Intestinal Mucosa and Protective Effects of CA in Rats

作　　者：严玉霖[1] 高洪[1] 卢琴[1] 王修庚[1] 赵汝[1] 陈玲[1] 

机构地区：[1]云南农业大学动物科学技术学院,云南昆明650201

出　　处：《动物医学进展》2015年第1期53-56,共4页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目(30560113);云南省现代农业生猪产业技术体系建设项目(云财农[2010]119号);云南省高校科技创新团队支持计划资助项目(云教科[2011]14号)

摘　　要：旨在揭示大肠埃希菌内毒素(ET)对大鼠小肠黏膜的结构、绒毛长度、上皮内淋巴细胞(IEL)的数量和分布的影响,并探讨多价阳离子A(CA)对上述指标的保护效应。选用72只140g^150g SPF级SD大鼠随机分为3组,即对照组、ET组和CA保护组,经相应处理后分别在3、4、8、12h采集十二指肠、空肠组织作为检测样本,制备病理组织切片,HE染色并利用图像分析系统进行分析。ET组十二指肠和空肠绒毛长度在3、4、8、12h均显著低于对照组和CA保护组(P<0.01),ET组十二指肠、空肠IEL数量在3、4、8、12h均显著低于对照组(P<0.01);CA保护组十二指肠和空肠IEL数量在4、8、12h均显著高于ET组(P<0.01)。结果显示,ET在不同程度上能够破坏小肠黏膜的正常组织结构,降低小肠绒毛长度,减少IEL的数量,从而影响小肠正常的吸收和免疫功能,而CA则能明显降低ET所导致的毒性作用,发挥其保护效应。This study was to explore the influence of endotoxin （ET） on small intestinal mucosa tissue and the protective effects of CA in rats.72 SD rats were randomly divided into three groups ：the control group, ET group and CA group,after appropriate treatment the duodenum,jejunum tissue were collected and tested at 3,4,8,12 h.The pathological slices of above tissues were prepared and stained by H.E.,then were analyzed by graphical analysis system.The length of the intestinal villi of the duodenum and jejunum in ET group were shorter than in control and CA group at 3,4,8,12 h （P〈0.01）.The quantity of IEL in the duodenum and jejunum in ET group were less than that of control and CA group at 3,4,8,12 h （P〈0.01）.The quantities of IEL in the duodenum and jejunum in CA group were more than that of ET group at 4,8,12 h （P〈0.01）.The results indicated that ET could destroy the normal structures of small intestine,reduce the length of the intestinal villi,and consequently affect the normal absorptive and im-mune function.However,CA has protective effects by significantly relieving the toxic effects of ET.

关 键 词：内毒素 阳离子A 小肠绒毛长度 上皮内淋巴细胞 大鼠 

分 类 号：S852.3[农业科学—基础兽医学]