3个甘蔗与斑茅远缘杂交后代BC_1的染色体遗传分析  

Genetic Analysis of Chromosome in 3 BC_1 Clones from the Distant Crossing Between Saccharum spp.and Erianthus arundinaceus

作　　者：黄永吉[1] 符成[2] 林炜乐[1] 刘少谋[2] 高嘉慧 邓祖湖[1] 黄忠兴[2] 林彦铨[1] 陈如凯[1] 

机构地区：[1]福建农林大学农业部福建甘蔗生物学与遗传育种重点实验室,福建福州350002 [2]广州甘蔗糖业研究所,广东广州510316

出　　处：《热带作物学报》2015年第1期53-58,共6页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金(No.30671329);国家甘蔗产业体系专项资金资助(No.CARS-20-1-5)

摘　　要：对3个甘蔗与斑茅远缘杂交后代BC1进行真实性鉴定及染色体核型分析,以探讨甘蔗与斑茅BC1的染色体传递方式。利用2对鉴定斑茅真实杂交后代的特异引物对3个甘蔗与斑茅BC1进行鉴定,采用根尖分生区细胞去壁低渗涂片法制片,显微拍照计数染色体数目,并进行染色体核型分析。3个BC1材料均为斑茅的真实杂交后代,崖城01-69体细胞染色体核型公式为2n=121=120 m+1 sm,其染色体按2n+n方式传递;崖城01-116的体细胞染色体核型公式为2n=122=118 m+4 sm,其染色体传递方式为2n+n;崖城01-134的体细胞染色体核型公式为2n=121=120 m+1 sm,其染色体传递为2n+n。推断甘蔗与斑茅BC1的染色体以2n+n的方式传递。To explore the chromosome transmission, the karyotypes of 3 BC1 clones from the distant crossing between Saccharum spp. and Erianthus arundinaceus were analyzed. 3 BC1 clones were identified using 2 pairs of specific primers of true E. arundinaceus progenies. Chromosomes were prepared according to cell wall degradation hypotonic smear method, the chromosomes number of 3 BC1 clones was calculated and the karyotypes of 3 BCI clones were analyzed. 3 BC1 clones were true progenies of E. arundinaceus. The somatic chromosome karyotypic type of YC01-69 was 2n=121=120 m＋1 sm, following 2n＋n transmission; The somatic chromosome karyotypic type of YC01-116 was 2n=122=118 m＋4 sin, following 2n＋n transmission; The somatic chromosome karyotypic type of YC01-134 was 2n=121=120 m＋l sin, following 2n＋n transmission. The 2n＋n transmission of BC1 clones from Saccharum spp. and E. arundinaceus can be resumed.

关 键 词：甘蔗 斑茅 核型分析 染色体传递方式 

分 类 号：S334.3[农业科学—作物遗传育种]