鸦胆子油乳治疗H22肝癌荷瘤小鼠的时辰药理学研究  

Chronopharmacology Study of Bruceolic Oil Emulsion for Treatment of H22 Tumor-bearing Mice

作　　者：李滨萍[1] 林曦[2] 廖嘉仪[2] 刘方乐[2] 熊天琴[2] 

机构地区：[1]广东省中医院药学部,广东广州510120 [2]广州中医药大学,广东广州510405

出　　处：《广州中医药大学学报》2015年第1期111-115,120,共6页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：广东省中医药局科研课题(编号:20121181)

摘　　要：【目的】研究鸦胆子油乳治疗H22肝癌荷瘤小鼠的时辰药理学的规律。【方法】制备H22肝癌荷瘤小鼠模型;造模成功的小鼠分组后,分别在正常光节律及异常光节律条件下进行治疗。治疗结束后计算肿瘤抑制率及脏器指数,测定肝、肾功能指标。【结果】在正常光节律和异常光节律下,各治疗组均能减小瘤体质量,降低瘤重指数,其中,正常光节律下的暗期前期给药组,异常光节律下的暗期前期给药组、暗期中期给药组,分别与相应的模型组比较,差异均有统计学意义(P<0.05)。正常光节律下,暗期前期(10 pm)给药组的肝脏指数显著下降、胸腺指数显著上升,与模型组比较差异有统计学意义(P<0.05);异常光节律下,暗期前期(7 am)给药组的脾脏指数较模型组显著上升,差异有统计学意义(P<0.05)。【结论】鸦胆子油乳注射液在正常光节律和异常光节律下均有一定抑制肿瘤的作用,药物在暗相期给药治疗效果更佳。Objective To investigate the regularity of chronopharmacology of bruceolic oil emulsion （ BOE） in treating H22 tumor-bearing mice. Methods The KM mice model bearing H22 hepatocellular carcinoma was established. And then the model mice were divided into groups according to the condition of illumination and＆nbsp;medicine intervention. After treatment for 10 days, the tumor-growth inhibitory rate, viscera indexes, and hepatic and renal function indexes were observed. Results Tumor mass and ratio of tumor mass to body mass were decreased in medication groups under the normal and disordered illumination. The tumor mass and ratio in mice treated with BOE before dark phase of normal illumination and disordered illumination, and during the dark phase of disordered illumination differed from those of the model group at the corresponding illumination phase （P〈0.05） . Under normal illumination, BOE-treated mice at 10 pm of dark phase had lower liver index but higher thymus index than the model group （P 〈 0.05） . Under disordered illumination, BOE-treated mice at 7 am of dark phase had higher spleen index than the model group （ P 〈 0.05） . Conclusion BOE exerts certain inhibition of H22 hepatocellular carcinoma under both normal illumination and disordered illumination, and the effect will be better when medication is given in dark phase period.

关 键 词：鸦胆子油乳/药理学 时辰药理学 肝癌/中药疗法 疾病模型  动物 小鼠 

分 类 号：R285.5[医药卫生—中药学]