果桑肥大性菌核病菌和油菜菌核病菌的交叉侵染、生物学特性及遗传关系  

Cross Infection, Biological Characteristics and Genetic Relationship between Pathogens of Hypertrophy Sorosis Sclerotenisis from Mulberry and Sclerotinia Stem Rot from Oilseed Rape

作　　者：吕蕊花[1] 金筱耘[2] 赵爱春[1] 吉洁 刘长英[1] 李军[1] 蒲龙 鲁成[1] 余茂德[1] 

机构地区：[1]西南大学生物技术学院/家蚕基因组生物学国家重点实验室,重庆400716 [2]西南大学农学与生物科技学院,重庆400716 [3]四川省南部县蚕桑局,四川南部637300

出　　处：《作物学报》2015年第1期42-48,共7页Acta Agronomica Sinica

基　　金：中央高校基本业务专项资金项目(XDJK 2014D016);国家公益性行业(农业)科研专项(201403064);国家现代农业产业技术体系建设专项(CARS-22)资助

摘　　要：分别以果桑肥大性菌核病菌和油菜菌核病菌的子囊孢子交叉接种油菜和果桑。结果表明,杯盘菌子囊孢子能够侵染油菜,同样,核盘菌子囊孢子能够侵染果桑;在受侵染的桑椹上2种病菌均产生分生孢子梗和分生孢子,而在油菜上不产生。显微镜观察,杯盘菌的子囊盘外囊被切面为圆胞组织结构,核盘菌为角胞组织结构。SRAP分子标记和聚类结果表明,采自西南地区果桑上的杯盘菌和油菜上核盘菌基本各自聚在一类,其中一个杯盘菌分离物和一个核盘菌分离物聚在一类,表现特别。2种寄主上的病菌子囊孢子能相互侵染,因而果桑和油菜不能间套种植。The cross infection was studied between ascospores of Ciboria shiraiana causing hypertrophy sorosis sclerotenisis in mulberry and Sclerotinia sclerotiorum causing Sclerotinia stem rot in oilseed rap. Inoculation results showed that ascospores of C. shiraiana infected oilseed rape, and ascospores of S. sclerotiorum infected sorosis similarly. Conidia and conidiophores appeared in the infected sorosis by C. shiraiana and S. sclerotiorum, but did not appear in the infected oilseed rape. The cross section showed a structure of capsule ascus round cell in C. shiraiana under microscope, but the texture angularity in S. sclerotiorm. Clustering based on SRAP of isolates from southwest areas showed C. shiraiana isolates from sorosis was classified into one group, and S. sclerotiorm isoplates from oilseed rape into a second group, with an except where one C. shiraiana isolate and one S. sclerotiorm isolate were clustered into an additional group. Cross infection of the two host plants by these two pathogen ascospores suggests that intercropping or interplanting of mulberry and oilseed rape is not feasible where the two pathogens/diseases exist.

关 键 词：果桑 油菜 菌核病菌 交叉侵染 SRAP分子标记 

分 类 号：S432.4[农业科学—植物病理学]