小麦–中间偃麦草渗入系抗白粉病基因PmCH7124的分子定位  

Molecular Mapping of Powdery Mildew Resistance Gene Pm CH7124 in a Putative Wheat–Thinopyrum intermedium Introgression Line

作　　者：李建波[1] 乔麟轶[1,2] 李欣[2] 张晓军[2] 詹海仙[2] 郭慧娟[2] 任永康[2] 畅志坚[2] 

机构地区：[1]山西大学研究生院,山西太原030006 [2]山西省农业科学院作物科学研究所/农业部黄土高原作物基因资源与种质创制重点实验室,山西太原030031

出　　处：《作物学报》2015年第1期49-56,共8页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(31171839);山西省国际科技合作计划项目(2013081007);山西省科技攻关项目(20130311001-5)资助

摘　　要：小麦新种质CH7124由八倍体小偃麦TAI8335与高感白粉病小麦品种晋麦33杂交后代衍生而来,在苗期对白粉病菌株E09、E20、E21、E23、E26、Bg1和Bg2表现免疫或高抗,抗病表现与TAI8335及其野生亲本中间偃麦草相似。基因组原位杂交未检测到CH7124含有外源染色体信号。利用CH7124与感病亲本SY95-71和绵阳11的杂交群体接种鉴定和遗传分析证实,CH7124成株期对E09的抗性由1对显性核基因控制,暂命名为Pm CH7124。采用分离群体分组分析法(bulked segregant analysis,BSA)对SY95-71/CH7124的F6群体进行SSR标记扫描,发现抗性基因Pm CH7124与5对SSR标记连锁,与两翼邻近标记Xgwm501和Xbarc101的遗传距离分别为1.7 c M和4.5 c M。利用中国春缺体–四体和双端体材料,将Pm CH7124及其连锁标记定位在小麦2B染色体长臂上。通过分析2BL上其他抗白粉病基因的抗谱、抗性来源、物理图谱位置以及连锁标记在Pm CH7124作图群体中的多态性,认为Pm CH7124不同于2BL上已知的抗白粉病基因Pm6、Pm33、Pm JM22、Ml Zec1、Ml AB10和Ml LX99。Wheat introgression line CH7124 derived from a cross between wheat–Thinopyrum intermedium line TAI8335（resistant to powdery mildew） and common wheat variety ＂Jinmai 33＂（susceptible to powdery mildew） exhibits immunity to Blumeria graminis f. sp. tritici（Bgt） pathotypes E09, E20, E21, E23, E26, Bg1, and Bg2 at the seedling stage. The Bgt resistance in CH7124 is similar to that in TAI8335 and its wild parent Th. intermedium. However, no Th. intermedium chromatin has been detected according to genome in situ hybridization（GISH） assay. In this study, we determined the single dominant Bgt resistance gene in CH7124, tentatively designated Pm CH7124, using populations derived from SY95-71/CH7124（F6） and CH7124/Mianyang 11（F1and F2）. Five SSR markers（Xgwm47, Xgwm120, Xwmc332, Xgwm501, and Xbarc101） were identified to be codominant with Pm CH7124 according to bulked segregant analysis, and the closely flanking markers were Xgwm501 and Xbacr101 with genetic distances of 1.7 c M and 4.5 c M, respectively. The target resistance gene was chromosomally located on 2BL with Chinese Spring nulli-tetrasomicand ditelosomic lines. We primarily consider that Pm CH7124 is a new Bgt resistance gene because its resistance spectrum, origin, chromosomal location, and linked markers are different from those of the known Bgt resistance genes, such as Pm6, Pm33, Pm JM22, Ml Zec1, Ml AB10, and Ml LX99.

关 键 词：小麦–中间偃麦草渗入系 白粉病抗性 GISH SSR标记 连锁图谱 

分 类 号：S512.1[农业科学—作物学]