抑郁复合皮肤创伤对创面微血管及成纤维细胞生长的影响  

Effect of depression combined with skin injury on wound capillary angiogenesis and fibroblast growth

作　　者：周明芳[1] 程天民[2] 冯正直[3] 

机构地区：[1]第三军医大学护理学院临床护理学教研室,重庆400038 [2]第三军医大学军事预防医学院全军复合伤研究所,重庆400038 [3]第三军医大学心理学院,重庆400038

出　　处：《重庆医学》2015年第2期145-147,共3页Chongqing medicine

基　　金：军队"十一五"科技攻关项目(06G076)

摘　　要：目的：观察抑郁复合皮肤创伤对创面新生微血管及成纤维细胞消长的影响。方法根据建模方法将144只SD大鼠随机分为单创组、创伤复合抑郁组、抑郁复合创伤组，每组48只，制备抑郁模型。分别采用CD34、Vimentin抗体行免疫组织化学标记血管内皮细胞及成纤维细胞，高倍显微镜下计数。结果伤后第3天单创组和创伤复合抑郁组大鼠的创面微血管数显著高于抑郁复合创伤组（P＜0．05），第21天时各组间微血管计数无显著差异；伤后第3天抑郁复合创伤组创面成纤维细胞虽少于单创组和创伤复合抑郁组，但差异无统计学意义（P＞0．05）；创伤后第18～21天，3组间成纤维细胞数量比较，差异有统计学意义（P＜0．05）。结论抑郁复合皮肤创伤时，创面肉芽组织中微血管及成纤维细胞的生长合成可被显著抑制，使伤口愈合延缓。Objective To explore the effect of depression combined with skin injury on wound capillary angiogenesis and fibro‐blast growth .Methods Totally 144 Sprague‐Dawley rats were randomly and equally divided into 3 groups：simple wound group ,de‐pression combined wound group ,wound combined depression group ,48 mice in each of the 3 groups .Immunohistochemical staining was used to label vascular endothelial cell and fibroblast with CD34 and Vimentin antibody respectively ,then counted by the high resolution microscope .Results Compared with other 2 groups ,the number of micro vascular and fibroblast in the wound granula‐tion tissue were lower in depression combined with mechanical skin injury group at the first stage（3 days after injury）（P0 .05） .There was significant difference between 3 groups in the num‐ber of fibroblast during the 18th day and 21st day（P〈0 .05） .Conclusion Depression combined with skin injury could significantly inhibit the capillary angiogenesis and fibroblast growth in the wound granulation tissue ,thereby delaying wound healing .

关 键 词：抑郁 成纤维细胞 微血管 创面愈合 

分 类 号：R641[医药卫生—外科学]