大量失血对老年大鼠空间学习记忆能力的影响  

The influences of massive hemorrhage on spatial learning and memory in aged rats

作　　者：吕一冬 刘秀珍[2] 王宏月[1] 魏昌伟[2] 王卓强[2] 陈剑[2] 

机构地区：[1]江苏省麻醉学重点实验室,江苏徐州221004 [2]解放军第309医院麻醉科,北京100091

出　　处：《重庆医学》2015年第2期158-160,共3页Chongqing medicine

基　　金：总参军事医学和老年病科研基金项目(ZCWS14C20)

摘　　要：目的探讨大量失血对SD老年大鼠空间学习记忆能力的影响。方法将56只SD老年大鼠分为空白组(B组,n=8)、对照组(C组,n=24)和大量失血组(H组,n=24)。B组不做任何处理,C组对老年大鼠麻醉后,游离股动脉并结扎、缝合。H组对老年大鼠麻醉后,先游离股动脉然后行股动脉穿刺、放血,再行股动脉结扎、缝合。用Morris水迷宫检测3组老年大鼠不同时段的空间学习记忆能力。结果 H组术后1、3、7d的逃避潜伏期分别为:(29.93±7.93)、(34.56±6.74)、(15.47±6.42)s。与B组逃避潜伏期(12.56±3.08)s比较,H组老年大鼠的逃避潜伏期在大量失血后1、3d差异均有统计学意义(P<0.05),大量失血后7d差异无统计学意义(P>0.05)。C组老年大鼠在术后1、3、7d的逃避潜伏期分别为:(15.74±5.86)、(15.61±2.56)、(13.56±4.61)s,与B组逃避潜伏期(12.56±3.08)s比较,差异均无统计学意义(P>0.05)。结论大量失血导致了老年大鼠空间学习记忆能力的损害。Objective To research the influences of massive hemorrhage on spatial learning and memory ability in elderly SD rats .Methods Fifty six aged SD rats were randomly divided into 3 groups：blank group （B group ,n=8） ,control group （C group , n=24） ,and hemorrhage group （H group ,n=24） .B group was not given any intervention .C group received femoral artery ligation and was sutured under general anesthesia .H group underwent femoral artery puncture phlebotomy ,and then the rats were sutured . Morris water maze was used to test the spatial working ability .Results The escape latency of H group on postoperative day 1 ,3 and 7 were（29 .93 ± 7 .93）s ,（34 .56 ± 6 .74）s and （15 .47 ± 6 .42）s respectively .Compared with B group（12 .56 ± 3 .08）s ,these re‐sults indicated the spatial learning and memory of H group was destroyed after surgery 1 d and 3 d （P0 .05） .The escape latent periods to platform observed in C group rats on day1 ,3 and 7 after operation were（15 .74 ± 5 .86）s ,（15 .61 ± 2 .56）s and （13 .56 ± 4 .61）s .Compared with B group [（12 .56 ± 3 .08）s] ,these results indicated that there was no significant difference 7 （P〉0 .05） .Conclusion The findings of this study indicate that massive hemorrhage of old rats may destroy the spatial learning and memory .

关 键 词：出血 记忆 老年大鼠 MORRIS水迷宫 

分 类 号：R614[医药卫生—麻醉学]