基于UPLC/Q-TOFMS技术的白术药材化学成分快速识别研究  

Rapid Identification of Components fromAtractylodis macrocephalae Rhizoma Based on UPLC/Q-TOF MS

作　　者：钟艳梅[1] 冯毅凡[1] 郭姣 

机构地区：[1]广东药学院中心实验室,广东广州510006 [2]国家中医药管理局高脂血症"调肝降脂"重点研究室,国家中医药管理局"脂代谢"三级实验室,广东省代谢性疾病中医药防治重点实验室,广东广州510006

出　　处：《质谱学报》2015年第1期72-77,共6页Journal of Chinese Mass Spectrometry Society

基　　金：广东省自然科学基金团队项目(10351022401000000);广东省教育部产学研结合项目(2011B090400379)资助

摘　　要：建立了超高效液相色谱-串联四极杆飞行时间质谱(UPLC/Q-TOF MS)法快速鉴定白术生药材中的多种化学成分。采用Aquity UPLC/Q-TOF micro联用仪,以水-乙腈流动相梯度洗脱,ESI+模式采集数据,Masslynx4.1软件分析处理数据。实验分析了白术药材中的24个峰信号,鉴定了其中的20种化学成分。结果表明,应用UPLC/Q-TOF MS技术能够快速鉴定白术药材的化学成分,是中药成分分析的有效手段。The components of Atractylodis rnacrocephalae Rhizoma were identified by ultra-high performance liquid chromatography with tandem quadrupole time-of-flight mass spectrometry （UPLC/Q-TOF MS）. Using Aquity UPLC/Q-TOF micro system gradient elute with water-acetonitrile mobile phase under positive mode of ESI; data were analyzed by Masslynx4.1 software. Twenty four peaks in chromatogram of Atrac- tylodis macrocephalae Rhizoma were analyzed, and 20 kinds of components were identi- fied. Rapid identification of components from Atractylodis macrocephalae Rhizoma was carried out by UPLC/Q-TOF MS, which will be an effective device in analysis of com- positions research in traditional Chinese medicine.

关 键 词：超高效液相色谱-串联四极杆飞行时间质谱(UPLC/Q-TOF MS) 化学成分 白术 

分 类 号：O657.63[理学—分析化学] R284.1[理学—化学]