鼓槌石斛杂交育种及组培育苗技术研究  

Studies on Cross Breeding of Dendrobium chrysotoxum and Its Seedlings in vitro

作　　者：高燕[1] 李泽生[1] 李桂琳[1] 白燕冰[1] 耿秀英[1] 李薇莎[1] 周侯光[1] 

机构地区：[1]云南省德宏热带农业科学研究所,云南瑞丽678600

出　　处：《热带农业科技》2015年第1期15-19,23,共6页Tropical Agricultural Science & Technology

基　　金：农业部物种资源保护财政专项"瑞丽石斛种质圃"(13RZZY-27)

摘　　要：通过对鼓槌石斛野生种质人工杂交获得蒴果,再以蒴果种子为外植体进行组培快繁和假植育苗试验,结果表明:人工杂交授粉结果率达65.4%;种子萌发及原球茎诱导培养基为1/2Mb+水解乳蛋白1g·L-1+2.5%白糖,萌发率达95%;增殖分化培养基为3/4Mb+NAA0.2 mg·L-1+6-BA0.1 mg·L-1+8%马铃薯泥+4%香蕉泥+3%白糖,增殖倍数达12.6倍;壮苗生根培养基为3/5Mb+NAA0.5 mg·L-1+IBA 0.1 mg·L-1+5%马铃薯泥+7%香蕉泥+0.1%AC+2%白糖,生根率达100%,移栽成活率达96%以上。Artificial hybridization of wild Dendrobium chrysotoxum was made to obtain the capsule. Results from experi- ment of rapid propagation in vitro and transplant seedlings indicated that fruit set following cross pollina- tion would reach to 65.4%. Seed germination in medium of 1/2 Mb ＋ 1 g ·L-1hydrolytic lactalbumin ＋ 2.5% of sug- ar would be 95%, and proliferation rate in medium of 3/4 Mb＋ NAA0.2 mg·L-1＋6-BA0.1 mg· L-1＋ 8% of potato puree＋ 4% of banana puree＋3% of sugar would increase by 12.6 times, the rooting is 100 percentage in medium of 3/5 Mb＋ NAA0.5 mg·L-1＋ IBA0.1 mg· L-1＋5% of potato puree ＋ 7% of banana puree ＋0.1% AC ＋2% of sugar with 96% of surviv- al rate or above.

关 键 词：鼓槌石斛 杂交育种 组培 假植育苗 

分 类 号：S682.31[农业科学—观赏园艺]