小麦生长素结合基因TaABP1-D的表达与染色体定位  

Expression and Chromosomal Localization of Wheat TaABP1-D Gene

作　　者：李欣[1] 张晓军[1] 詹海仙[1] 郭慧娟[1] 李建波[2] 畅志坚[1] 乔麟轶[1,2] 

机构地区：[1]山西省农业科学院作物科学研究所/农业部黄土高原作物基因资源与种质创制重点实验室,太原030031 [2]山西大学研究生院,太原030006

出　　处：《植物遗传资源学报》2015年第1期107-112,共6页Journal of Plant Genetic Resources

基　　金：国家自然科学基金项目(31171839);山西省国际科技合作计划项目(2012081006-2;2013081007);山西省科技攻关项目(20130311001-5);山西省回国留学人员科研资助项目(2012-102)

摘　　要：生长素影响了植物生长发育的诸多过程。生长素结合蛋白ABP1(auxin binding protein)作为一种生长素受体,在质膜上生长素诱导的快速反应中起重要作用。小麦中已经克隆获得了TaABP1-D,但其在细胞中的作用位置以及在染色体的定位情况仍不明确。本研究利用洋葱表皮细胞瞬时表达系统对小麦生长素结合基因TaABP1-D进行亚细胞定位,结果表明TaABP1-D蛋白为膜蛋白,存在于细胞质和细胞膜中;同时利用中国春缺体-四体材料和信息学方法,将TaABP1-D定位在小麦5D染色体长臂的近着丝粒位置上,距两侧EST标记BE490079和BE405060的遗传距离分别为0.51 c M和0.28 c M。Auxin affects many processes of plant growth and development. As an auxin receptor, auxin binding protein（ ABP1 ） plays an important role in the rapid reactions induced by auxin on the plasma membrane. The ABP1 in common wheat, named TaABP1-D, was previously cloned, but its chromosome map and the distribution in the cell are still not clear. In this study, we located the TaABP1-D on the cytoplasm and cell membrane by transiently ex- pressed system in onion epidermal cells, which indicated that TaABP1-D protein was a membrane protein. In addi- tion, using Chinese Spring nullitetrasomic-tetrasomic lines and bioinformatics methods,we located the TaABP1-D in the perieentromerie region of the long arm of wheat chromosome 5D. EST markers BE490079 and BE405060 flanked TaABP1-D by O. 51 cM and 0.28 cM, respectively.

关 键 词：小麦 TaABP1-D 亚细胞定位 信息学 染色体定位 

分 类 号：S512.1[农业科学—作物学]