云南野生稻抗白叶枯病类Xa21基因的鉴定  

Identification of Resistance Gene Xa21 in Yunnan Wild Rice

作　　者：李维蛟[1] 陈玲[1] 殷富有[1] 张敦宇[1] 程在全[1] 

机构地区：[1]云南省农业科学院生物技术与种质资源研究所/云南省农业生物技术重点实验室/农业部西南作物基因资源与种质创制重点实验室,昆明650223

出　　处：《植物遗传资源学报》2015年第1期113-118,共6页Journal of Plant Genetic Resources

基　　金：国家自然科学基金(U1302265;3160067;31460478);云南省高端人才引进项目(2012H002);农业部行业专项(201003021)

摘　　要：水稻白叶枯病是水稻生产上的主要细菌病害之一。从野生稻中发掘优异的水稻白叶枯病抗性材料,可以拓宽栽培稻抗白叶枯病遗传基础。经过温室接菌鉴定和PCR标记分析,对云南野生稻进行Xa21基因的检测鉴定。温室接菌鉴定表明,云南野生稻对广谱致病小种PX099及云南强致病菌Y8具有较好的抗性能力,特别是疣粒野生稻对致病菌株达到免疫程度;PCR标记分析表明,云南野生稻不含有Xa21基因,但含有与Xa21基因某些区域同源的片段。本研究结果为寻找新的抗源材料及快速发掘利用云南野生稻中的抗白叶枯病基因提供理论依据。Rice bacterial blight is one of the major bacterial diseases of rice production. Rice bacterial blight resistance materials developed from wild rice may increase rice resistance resource. Xa21 gene in Yunnan wild rice was detected by inoculation identification and PCR marker analysis. The results of inoculation identification showed that Yunnan wild rice were excellently resistant to PX099 and YS, especially, O. meyeriana was immunity. The re- sults of PCR marker analysis showed that Yunnan wild rice didn＇t contain Xa21 gene, nevertheless, they carried with Xa21 homologous fragment gene. It would lay the foundation for finding out new bacterial blight resistance re- source and exploring new resistance gene.

关 键 词：水稻白叶枯病 野生稻 XA21基因 

分 类 号：S511.9[农业科学—作物学]