基于SSR和ISSR的鄱阳湖流域野生菰资源的遗传多样性分析  

Genetic Diversity of Zizania latifolia Griseb. from Poyang Lake Basin Based on SSR and ISSR Analysis

作　　者：王惠梅[1] 吴国林[2,3] 江绍琳[3] 黄奇娜[1] 奉保华[1] 黄长干[3] 江绍玫[2] 吴建利[1] 

机构地区：[1]中国水稻研究所,杭州310006 [2]江西财经大学,南昌330045 [3]江西农业大学,南昌330045

出　　处：《植物遗传资源学报》2015年第1期133-141,共9页Journal of Plant Genetic Resources

基　　金：国家自然科学基金(31160271);江西省科技支撑项目(20133BBF6005)

摘　　要：用SSR和ISSR标记对鄱阳湖流域30个野生菰居群的遗传多样性与遗传结构进行了分析。筛选出的19对SSR引物共扩增出多态性条带253条,平均多态性条带比率（PPB）为91.67%,Nei＇s基因多样性指数（He）和平均Shannon信息指数（I）分别为0.2712和0.4144,遗传相似性系数（GS）为0.5590-0.8368,遗传距离（GD）的变化范围为0.1632-0.4410;筛选出的14个ISSR标记引物共扩增出83条条带,平均多态性条带比率（PPB）为78.29%,Nei＇s基因多样性指数（He）和平均Shannon信息指数（I）分别为0.2386和0.4174,遗传相似性系数（GS）为0.5132-0.9342,遗传距离（GD）变化范围为0.0658-0.4868。根据SSR和ISSR基因型数据,采用UPGMA法分别在阈值为0.698和0.728时可将30个野生菰居群聚为3类。可能受人为、水流、动物活动、风等多种因素的影响,居群间的亲缘关系与地理分布无明显相关性。本研究表明,鄱阳湖流域野生菰居群间SSR和ISSR基因型的多样性丰富,居群间的这种遗传差异或变异,对该地区乃至更大范围内野生菰的遗传进化、基因资源的开发利用和种质资源的保护有着重要意义。Genetic diversity of 30 local wild rice populations of Zizania latifolia collected from the Poyang Lake valley was investigated using SSR （ simple sequence repeat） and ISSR （ inter-simple sequence repeat） markers. 253 bands were generated from 19 pairs of informative SSR primers with an average polymorphism percentage bands （PPB） of 91.67%. The average index of Nei＇s genetic diversity （He）and Shannon＇s information （I）were 0.2712 and 0.4144, respectively. The genetic similarity index（GS） and genetic distance（GD） ranged from 0. 5590 - 0. 8368 and 0.1632 - 0. 4410, respectively. In comparison, with 14 informative ISSR primers, 83 bands were amplified with an average PPB of 78.29%. The average of He and I were O. 2386 and 0.4174, respectively. The GS and GD ranged from 0. 5132 -0.9342 and 0. 0658 -0. 4868, respectively. Cluster analysis showed that 30 wild populations of Z. latifolia could be classified into three groups respectively at the threshold of 0. 698 and 0. 728 based on SSR and IS- SR genotypes using the UPGMA analysis. There was no significant association between the phylogenetic relationship and the geographic locations in the Z. latifolia populations which might be due to human activity, water flow, animalactivity,wind, etc. The results indicated that the wild populations of Z. latifolia from the Poyang Lake valley had a rich genetic diversity in SSR and ISSR genotype and thus it was of great importance to explore genetic evolution and gene resources and to preserve the local Z. latifolia populations in this area and expanded area.

关 键 词：菰 居群 遗传多样性 SSR标记 ISSR标记 

分 类 号：Q943[生物学—植物学]