生物信息学预测植物miRNAs的方法  

Bioinformatics Prediction of MicroRNAs in Plant

作　　者：张廷婷[1] 胡述浩[2] 闫彩霞[1] 赵小波[1] 单世华[1] 姜林平[3] 

机构地区：[1]山东省花生研究所,青岛266100 [2]山东大学威海分校,威海264209 [3]海阳市农业技术推广站,山东海阳265100

出　　处：《植物遗传资源学报》2015年第1期147-150,157,共5页Journal of Plant Genetic Resources

基　　金：现代农业产业技术体系建设专项(CARS-14);山东省现代农业产业技术体系项目(SDAIT-05-011-03);山东省农业生物资源创新利用研究课题

摘　　要：MicroRNA又称miRNA,是一类广泛存在于动植物体内、大小为21-25nt左右的内源性非编码单链小分子RNA。在植物体中,miRNA主要负责调控转录后基因的表达,在机体发育、生长和应答胁迫方面发挥着重要的调节作用。近年来,miRNA已成为分子生物学领域的研究热点。本文介绍了几种发现和研究miRNA的方法,重点阐述了生物信息学预测miRNA方法的流程和策略,并对各过程中采用的方法及信息学软件的优劣性进行比较和分析,为预测miRNA提供新的思路。MicroRNAs （miRNAs） are endogenous non-coding single-strand molecules with 21-25 nucleotides in length, and they are widely existed in plants and animals. In plant, miRNAs have been confirmed that they nega- tively regulate gene expression at the post-transcriptional level and play an important regulatory role in plant growth and development, and response to various biotic and abiotic stresses. In recent years, miRNA has become the hots- pot in modem molecule biology. In this paper, several methods for identification and charactrization of plant miR- NAs were introduced, and further the processes and strategies for miRNA bioinformatics prediction were reviewed with emphasis, as well as investigation and analysis of some bioinformatics softwares. This paper will provide new ideas for miRNA identification in plant.

关 键 词：生物信息学 植物 miRNA预测 

分 类 号：Q943[生物学—植物学]