大豆PEBP基因家族的初步分析  

Preliminary Analysis of the PEBP Gene Family in Soybean(Glycine max)

作　　者：张礼凤[1] 徐冉[1] 张彦威[1] 戴海英[1] 王彩洁[1] 李伟[1] 张军[1,2] 

机构地区：[1]山东省农业科学院作物研究所,济南250100 [2]滨州职业技术学院,山东滨州256603

出　　处：《植物遗传资源学报》2015年第1期151-157,共7页Journal of Plant Genetic Resources

基　　金：国家转基因重大专项(2013ZX08004);国家现代农业产业技术体系(CARS-04-CES18)

摘　　要：磷脂酰乙醇胺结合蛋白(PEBP,phosphatidyl ethanolamine-binding protein)基因家族在动物、植物和微生物中广泛存在,在控制植物开花和种子休眠中起重要作用。本研究对大豆PEBP基因家族进行了分析,发现了27个大豆PEBP基因的候选序列,其中16个具有完整PEBP结构域的全长序列被认为是大豆Gm PEBP家族基因。Gm PEBP基因分布在9条染色体上,基因结构高度保守。通过系统发生分析,可将大豆Gm PEBP基因家族成员分为FT-like、TFL1-like和MFT-like 3个亚族,并且发现Gm PEBP家族成员数目按照大豆物种特异性的方式进行了扩张。对重复基因的Ks分析表明,绝大多数重复基因主要由5900万年前和1300万年前的大豆基因组复制所致。The PEBP gene family encoding proteins with similarity to phosphatidyl ethanolamine binding pro- teins was found in the animals, plants, and microbes. The PEBP genes played important roles on regulation of flow- ering time and seed dormancy. We presented here a systematic analysis of the PEBP gene family in soybean （ Gly- cine max）. At the level of genome-wide screening, we identified 27 PEBP candidate genes from soybean and consid- ered 16 genes with full length of coding sequence as GmPEBP genes. Sequence analysis showed the GmPEBP genes had conserved genomic structures and were distributed on 9 soybean chromosomes. Phylogenetic analysis showed that the GmPEBP family could be grouped into three subfamilies, named FT-like, TFLl-like, and MFI＇-like, and suggested that species-specific expansion contributed to the evolution of this family. Analysis of synonymous substi- tution rates indicated most duplicated pairs were from the whole genome duplication occurred at 59 and 13 million years ago.

关 键 词：大豆 PEBP基因家族 进化分析 

分 类 号：S565.1[农业科学—作物学]