腹主动脉阻断合并脂多糖内毒素攻击时大鼠心脏 TNF-α、SOD 的表达研究  

Expressions of TNF-αand SOD in cardiac tissues of rats with abdominal aorta champing and LPS attack

作　　者：易汉荣[1] 徐洁[1] 周青山[1] 

机构地区：[1]武汉大学人民医院重症医学科,430060

出　　处：《临床外科杂志》2014年第11期828-830,共3页Journal of Clinical Surgery

基　　金：湖北省自然科学基金资助项目（编号：2009CDB247）

摘　　要：目的：观察大鼠腹主动脉阻断术后合并脂多糖内毒素（LPS）攻击时心脏组织的病理改变及心脏组织中 TNF-α、SOD1、SOD2表达的变化。方法将 Wistar 大鼠40只随机分为4组：假手术组（S 组）；腹主动脉阻断／开放组（I ／R 组）；内毒素组（LPS 组）；双重打击组（I ／R ＋LPS 组）。所有动物均于腹主动脉阻断20 min．开放后8h 处死，取心脏组织行 HE 染色观察病理损害，免疫组化检测各组心脏 TNF-α、SOD1及 SOD2的表达变化。结果①双重打击组死亡率最高。②心脏组织病理学显示：S 组：染色均匀，心肌组织形态正常，心肌纤维排列规则，未见明显的病理改变；LPS组：心肌组织紊乱，心肌细胞略肿胀，肌间隙内可见红细胞漏出、炎性细胞浸润；I ／R 组：心肌组织紊乱，少数心肌变性、溶解，部分心肌纤维断裂，毛细血管充血、扩张，肌间隙内可见红细胞漏出、炎性细胞浸润，损伤程度较 LPS 组略重；I ／R ＋LPS 组：心肌细胞明显肿胀，肌间隙增宽，小血管充血明显，内有大量红细胞聚集，肌间隙内可见大量红细胞漏出，纤维变性、坏死，肌间隙炎性细胞明显增多聚集、贴壁，甚至出现结构模糊的溶断现象。③免疫组织化学染色检测结果：TNF-α表达数量的多少依次是 S 组＜LPS 组＜I ／R 组＜I ／R ＋LPS 组，LPS 组与 I ／R 组之间差异无统计学意义，余各组之间差异有统计学意义（P ＜0．05）；SOD1表达数量的多少依次 S 组＞LPS 组＞I ／R 组＞I ／R ＋LPS组，I ／R 组与 I ／R ＋LPS 组之间差异无统计学意义，余各组之间差异有统计学意义（P ＜0．05）；SOD2表达数量的多少依次是 S 组＞LPS 组＞I ／R ＋LPS 组＞I ／R 组，其中 LPS 组、I ／R 组与 I ／R ＋LPS 组之间差异无统计学意义，余各组之间差异有统计学意义（P ＜0．05）。结论大鼠在腹主动脉阻断／开放合�Objective To observe the pathomorphological changes and expressions of TNF-α, SOD1 and SOD2 in cardiac tissues of rats with abdominal aorta champing（AAC）and LPS attack.Methods Forty Wistar rats were randomly divided into four groups：sham operated group （Group S）,ischemia reperfusion with AAC group（Group I /R）,intraperitoneal injection of endotoxin group（Group LPS）and AAC ＋LPS group（Group I /R ＋LPS）.Animals were sacrificed after 20 minutes of AAC and 8 hours of reperfusion.Cardiac tissues were observed with HE staining for pathmmorphological changes,and detection of TNF-α,SOD1 and SOD2 expressions were tested by immunohistochemistry.Results The mortality rate of Group I /R ＋LPS was highest.HE staining of the cardiac tissues revealed that the pathological changes of myocardial tissue were not obvious in Group S;myocardial cells were disordered and slightly swollen and red blood cell and inflammatory cells were observed in the intramuscular space of the Group LPS;these injuries were more serious in the Group I /R;the injuries of cardiac tissues were most serious in Group I /R ＋LPS.The results of immunohistochemical staining revealed that TNF-αexpression level of cardiac tissue increased as followings：Group S,Group LPS,Group I /R and Group I /R ＋LPS.Significant differences were observed among groups,except for the difference between Group LPS and Group I /R.The expression level of SOD1 decreased as followings：Group S,Group LPS,Group I /R and Group I /R ＋LPS. Significant differences were observed among groups,except for the difference between Group I /R and Group I /R ＋LPS.The expression level of SOD2 decreased as followings：Group S,Group LPS,Group I /R ＋LPS and Group I /R.Significant differences were observed among groups,except for the differences between Group LPS and I /R and Group I /R ＋LPS.Conclusion The mortality in rats with sepsis after abdominal aorta clamping is high.Obvious myocardial morphological changes and injuries exist either in ischemia reperfusion or

关 键 词：缺血再灌注 脓毒症 心脏 TNF-Α 超氧化物歧化酶 

分 类 号：R542.52[医药卫生—心血管疾病]